Mouse IL-18/IL-1F4 ELISA

R&D Systems | Catalog # 7625

R&D Systems
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Serum (30 µL)

Sensitivity

25 pg/mL

Assay Range

25.6-1000 pg/mL
View all IL-18/IL-1F4 ELISA Kits »

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1 gamma, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.

Long Name

Interleukin 18

Alternate Names

IGIF, IL-1F4, IL-1g, IL18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Gene Symbol

IL18

Additional IL-18/IL-1F4 Products

Product Documents for Mouse IL-18/IL-1F4 ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-18/IL-1F4 ELISA

For research use only

Citations for Mouse IL-18/IL-1F4 ELISA

Customer Reviews for Mouse IL-18/IL-1F4 ELISA (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse IL-18/IL-1F4 ELISA?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Mouse IL-18/IL-1F4 ELISA
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 03/15/2023
    Mouse IL-18/IL-1F4 ELISA 7625
  • Mouse IL-18/IL-1F4 ELISA
    Name: Sebastien Fauteux
    Sample Tested: Serum and Plasma
    Verified Customer | Posted 07/13/2017
    You can shorten the incubation time if expected concentrations are near the maximum limit of the standards. Reproducible, efficient and recommendable.
    Mouse IL-18/IL-1F4 ELISA 7625

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits