Mouse IL-18 R alpha /IL-1 R5 Antibody

Catalog # Availability Size / Price Qty
MAB1216
MAB1216-SP

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Detection of IL‑18 R alpha /IL‑1 R5 in Mouse Splenocytes by Flow Cytometry.
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Mouse IL-18 R alpha /IL-1 R5 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑18 R alpha /IL‑1 R5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-1 RI, rmIL-1 RII, recombinant human (rh) IL-1 R3, rhIL-1 R4, recombinant rat (rr) IL-1 R6, rmIL-1 R7, rhIL-1 R8, rhIL-1 R9, or rmSIGIRR is observed.
Source
Monoclonal Rat IgG1 Clone # 112614
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑18 R alpha /IL‑1 R5
Ser19-Gly326
Accession # Q61098
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL‑18 R alpha /IL‑1 R5
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL‑18 Ra/IL‑1 R5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of IL‑18 R alpha /IL‑1 R5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL‑18 Ra/IL‑1 R5 Monoclonal Antibody (Catalog # MAB1216, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 R alpha/IL-1 R5

IL-18 R, also known as IL-18 R alpha, is a member of the IL-1 receptor family and has been designated IL-1 R5.

Long Name
Interleukin 18 Receptor alpha
Entrez Gene IDs
8809 (Human); 16182 (Mouse)
Alternate Names
CD218a; CD218a;CDw218a;IL18R1;IL18RA;IL-18Ralpha;IL-18R-alpha;IL18Ralpha2;IL1RRP;IL-1Rrp;Interleukin-18 receptor 1; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra

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Citation for Mouse IL-18 R alpha /IL-1 R5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Mapping of the full length and the truncated interleukin-18 receptor alpha in the mouse brain.
    Authors: Alboni S, Cervia D, Ross B, Montanari C, Gonzalez AS, Sanchez-Alavez M, Marcondes MC, De Vries D, Sugama S, Brunello N, Blom J, Tascedda F, Conti B
    J. Neuroimmunol., 2009-07-28;214(1):43-54.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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