Mouse IL-27 PE-conjugated Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.
  • Source
    Monoclonal Rat IgG2B Clone # 234205
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27
    Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
    Accession # O35228 (EBI3) & Q8K3I6 (p28)
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑27 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑27 PE‑conjugated Monoclonal Antibody (Catalog # IC1834P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of IL‑27 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL‑27 PE‑conjugated Monoclonal Antibody (Catalog # IC1834P) and Rat Anti-Mouse Integrin  alpha M/CD11b APC‑conjugated Monoclonal Antibody (Catalog # FAB1124A). Quadrant markers were set based on control antibody staining (Catalog # IC013P). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27
IL-27 is a non-covalent heterodimeric molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 33-34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23, and p28, the 28-30 kDa glycoprotein that is related to the p35 chain of IL-12. The mouse EBI3 gene encodes a 228 amino acid (aa) precursor that contains an 18 aa signal peptide and 210 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. EBI3 is known to heterodimerize with the IL-12 p35 subunit to form IL-35. Mature mouse EBI3 shares 91% and 61% aa sequence identity to mature rat and human EBI3, respectfully. The mouse p28 gene encodes a 234 aa precursor that contains a 28 aa signal sequence and 206 aa mature region. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Mature mouse p28 shares 89% and 70% aa sequence identity with mature rat and human p28, respectfully. IL-27 is expressed by monocytes, endothelial cells and dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. In addition, and in mouse but not human, p28 is secreted as a monomer and appears to activate the IL-6R complex. This is consistent with the observation that p28 also heterodimerizes with CLF1, and signals through the IL-6R:gp130 complex. IL-27 has both anti- and proinflammatory properties. As an anti‑inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL‑12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity that generates Th1 cells at the expense of Th2 and Th17 cells. Finally, IL-27 upregulates both MHC-II and chemokine (CXCL9; CXCL10) expression on vascular endothelium, suggesting a role for IL-27 in vascular inflammation.
  • Long Name:
    Interleukin 27
  • Entrez Gene IDs:
    246778 (Human); 246779 (Mouse)
  • Alternate Names:
    IL-27 p28 subunit; IL-27 subunit alpha; IL27; IL-27; IL27A; IL-27-A; IL27-A; IL27p28; IL30; interleukin 27; interleukin 30; interleukin-27 subunit alpha; MGC71873; p28IL-27A
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.

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 WB Mouse Anonymous 09/07/2017
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Summary

ApplicationWestern Blot
Sample TestedATDC5 mouse chondrogenic cell line
SpeciesMouse

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