Mouse IL-27 p28/IL-30 Antibody

Catalog # Availability Size / Price Qty
MAB1834
MAB1834-SP
Detection of IL‑27 in LPS-treated RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Mouse IL-27 p28/IL-30 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-27 in Western blots. This antibody recognizes the p28 subunit either as part of a p28/EBI-3 heterodimer or as a free subunit.
Source
Monoclonal Rat IgG2B Clone # 234205
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse IL‑27
Tyr19-Pro228 (EBI3) & Phe29-Ser234 (p28)
Accession # O35228 (EBI3) & Q8K3I6 (p28)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IL-27 p28/IL-30 (Catalog # 7430-ML)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-27 antibody in LPS-treated RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑27 in LPS-treated RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was unstimulated (light orange open histogram) or treated with 1 µg/mL LPS for 20 hours, then stained with Rat Anti-Mouse IL-27 p28/IL-30 Monoclonal Antibody (Catalog # MAB1834, dark orange filled histogram) or isotype control (Catalog # MAB0061, blue open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-27 p28/IL-30

Interleukin 27 (IL-27) is a novel heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI-3 (Epstein‑Barr virus‑induced gene 3). IL-27 binds TCCR/WSX-1 on naïve CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL‑12‑mediated Th1 cell development.

Long Name
Interleukin 27 p28
Entrez Gene IDs
246778 (Human); 246779 (Mouse)
Alternate Names
IL27 p28; IL-27 p28; IL27;IL-27;IL27A;IL-27A;IL27p28;IL-27p28;IL30;p28; IL-30

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