Mouse IL-3 R alpha /CD123 PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse IL-3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-3 R alpha  and recombinant mouse IL-3 R beta is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 151231
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-3 R alpha /CD123
    Accession # P26952
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑3 R alpha /CD123 in IL-3 Dependent DA3 Mouse Cell Line by Flow Cytometry. IL-3 dependent myeloid leukemia DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB983P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-3 R alpha/CD123

Interleukin 3 (IL-3) is a cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hematopoietic cells including pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to a specific cell surface receptor complex. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha  subunit and a beta  subunit. The alpha  subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). The alpha subunit is a member of the type I cytokine receptor family, type 5 subfamily (4,5). The mouse IL-3 R alpha extracellular domain (ECD) shares 42% and 29% amino acid sequence identity with rat and human IL-3 R alpha ECD, respectively.

  • References:
    1. Itoh, N. et al. (1990) Science 247:324.
    2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
    3. Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
    4. Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
    5. Broughton, S.E. et al. (2015) Cytokine 74:247.
  • Long Name:
    Interleukin 3 Receptor alpha
  • Entrez Gene IDs:
    3563 (Human); 16188 (Mouse)
  • Alternate Names:
    CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 receptor subunit alpha; IL3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 13  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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