Detects mouse IL-3 R alpha /CD123 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-3 R alpha and recombinant mouse IL-3 R beta is observed.
Monoclonal Rat IgG2A Clone # 151231
Protein A or G purified from hybridoma culture supernatant
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-3 R alpha /CD123 Ser17-Lys331 Accession # P26952
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of IL‑3 R alpha /CD123 in IL-3 Dependent DA3 Mouse Cell Line by Flow Cytometry.
IL-3 dependent myeloid leukemia DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB983P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-3 R alpha/CD123
Interleukin 3 (IL-3) is a cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hematopoietic cells including pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to a specific cell surface receptor complex. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and a beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). The alpha subunit is a member of the type I cytokine receptor family, type 5 subfamily (4,5). The mouse IL-3 R alpha extracellular domain (ECD) shares 42% and 29% amino acid sequence identity with rat and human IL-3 R alpha ECD, respectively.
Itoh, N. et al. (1990) Science 247:324.
Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
Broughton, S.E. et al. (2012) Immunol. Rev. 250:277.
Have you used Mouse IL-3 R alpha /CD123 PE-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.