Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8319A) and either (A) Rat Anti-Mouse Integrin alpha 2/CD49b PE‑conjugated Monoclonal Antibody (Catalog # FAB1740P) or (B) Rat IgG2B Phycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 2/CD49b
The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with beta 1 Integrin. The alpha 2 beta 1 Integrin is a collagen receptor that binds expressing cells to collagen and regulates collagen and collagenase secretion. Notably, this subunit has an "I" domain that is resistant to proteolysis. Over amino acids (aa) 27-1129, mouse Integrin alpha 2 shares 80% aa sequence identity with human Integrin alpha 2.
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