Mouse Integrin alpha 2/CD49b PE‑conjugated Antibody

Name: Mouse Integrin alpha 2/CD49b PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB1740P

R&D Systems | Catalog # FAB1740P

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Product Documents

Discontinued Product

FAB1740P has been discontinued. View all Integrin alpha 2/CD49b products.

Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG_2B Clone # 235033

View all Integrin alpha 2/CD49b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 2/CD49b
Tyr27-Thr1129
Accession # Q62469

Specificity

Detects mouse Integrin alpha 2/CD49b in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Integrin alpha 3, alpha 4, alpha 5, alpha E, or alpha V is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse Integrin alpha 2/CD49b PE‑conjugated Antibody

Detection of Integrin alpha 2/CD49b in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Mouse Anti-Mouse CD161/NK1.1 APC-conjugated Monoclonal Antibody (Catalog # FAB8319A) and either (A) Rat Anti-Mouse Integrin a2/CD49b PE-conjugated Monoclonal Antibody (Catalog # FAB1740P) or (B) Rat IgG_2BPhycoerythrin Isotype Control (Catalog # IC013P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin alpha 2/CD49b PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Integrin alpha 2/CD49b

The Integrin alpha 2 subunit, also known as CD49b and VLA-2 alpha subunit, forms a heterodimer with beta 1 Integrin. The alpha 2 beta 1 Integrin is a collagen receptor that binds expressing cells to collagen and regulates collagen and collagenase secretion. Notably, this subunit has an "I" domain that is resistant to proteolysis. Over amino acids
(aa) 27-1129, mouse Integrin alpha 2 shares 80% aa sequence identity with human Integrin alpha 2.