Mouse Integrin  alpha 3/CD49c Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2787G
Detection of Integrin  alpha 3/CD49c in XB2 Mouse Cell Line by Flow Cytometry.
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Mouse Integrin  alpha 3/CD49c Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 3/CD49c in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin alpha 2, rmIntegrin alpha 4, rmIntegrin alpha 5, rmIntegrin alpha E, rmIntegrin alpha M, rmIntegrin alpha V and rmIntegrin alpha X is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Integrin alpha 3/CD49c
Phe33-Glu993
Accession # Q62470
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

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Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a3/CD49c antibody in XB2 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 3/CD49c in XB2 Mouse Cell Line by Flow Cytometry. XB2 mouse teratoma keratinocyte cell line was stained with Goat Anti-Mouse Integrin a3/CD49c Alexa Fluor® 488-conjugated Polyclonal Antibody (Catalog # FAB2787G, filled histogram) or isotype control antibody (Catalog # IC108G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 3/CD49c

Integrin alpha 3, also known as CD49c and VLA-3 alpha, is a type I membrane protein that heterodimerizes with Integrin beta 1. alpha 3 beta 1 is a cell surface adhesion molecule that functions as a receptor for Laminin, Fibronectin, Collagen, Epiligrin, Thrombospondin and Chondroitin Sulfate Proteoglycan 4. The extracellular domain of human and mouse Integrin alpha 3 share approximately 87% amino acid sequence homology.

Entrez Gene IDs
3675 (Human); 16400 (Mouse)
Alternate Names
antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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