Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Integrin alpha 3/CD49c
Phe33-Glu993
Accession # Q62470

Specificity

Detects mouse Integrin alpha 3/CD49c in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinat mouse (rm) Integrin alpha 2, rmIntegrin alpha 4, rmIntegrin alpha 5, rmIntegrin alpha E, rmIntegrin alpha M, rmIntegrin alpha V and rmIntegrin alpha X is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Integrin  alpha 3/CD49c APC‑conjugated Antibody

Detection of Integrin alpha 3/CD49c antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 3/CD49c in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Goat Anti-Mouse Integrin a3/CD49c APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2787A, filled histogram) or isotype control antibody (IC108A, open histogram). Staining was performed using our View our Staining Membrane-associated Proteins protocol.

Detection of Integrin a3/CD49c antibody in XB2 Mouse Cell Line antibody by Flow Cytometry.

Detection of Integrin alpha 3/CD49c in XB2 Mouse Cell Line by Flow Cytometry.

XB2 mouse teratoma keratinocyte cell line was stained with Goat Anti-Mouse Integrin a3/CD49c APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2787A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Integrin  alpha 3/CD49c APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: XB2 mouse teratoma keratinocyte and C2C12 mouse myoblast cell lines

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 3/CD49c

Integrin alpha 3, also known as CD49c and VLA-3 alpha, is a type I membrane protein that heterodimerizes with Integrin beta 1. alpha 3 beta 1 is a cell surface adhesion molecule that functions as a receptor for laminin, fibronectin, collagen, epiligrin, thrombospondin and chondroitin sulfate proteoglycan 4. The extracellular domain of human and mouse Integrin alpha 3 share approximately 87% amino acid sequence homology.

Alternate Names

CD49c, ITGA3

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Gene Symbol

ITGA3

UniProt

Additional Integrin alpha 3/CD49c Products

Product Documents for Mouse Integrin  alpha 3/CD49c APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Integrin  alpha 3/CD49c APC‑conjugated Antibody

For research use only

Citations for Mouse Integrin  alpha 3/CD49c APC‑conjugated Antibody

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