Mouse Integrin alpha 3/CD49c APC-conjugated Antibody

FAB2787A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB2787A

Bulk Orders

Mouse Integrin alpha 3/CD49c APC-conjugated Antibody in Flow Cytometry

2 Images

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin alpha 3/CD49c APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Integrin alpha 3/CD49c in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinat mouse (rm) Integrin alpha 2, rmIntegrin alpha 4, rmIntegrin alpha 5, rmIntegrin alpha E, rmIntegrin alpha M, rmIntegrin alpha V and rmIntegrin alpha X is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Integrin alpha 3/CD49c
Phe33-Glu993
Accession # Q62470

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry

View Larger

Detection of Integrin alpha 3/CD49c in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Goat Anti-Mouse Integrin a3/CD49c APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2787A, filled histogram) or isotype control antibody (IC108A, open histogram). Staining was performed using our View our Staining Membrane-associated Proteins protocol.

Flow Cytometry

View Larger

Detection of Integrin alpha 3/CD49c in XB2 Mouse Cell Line by Flow Cytometry. XB2 mouse teratoma keratinocyte cell line was stained with Goat Anti-Mouse Integrin a3/CD49c APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2787A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 3/CD49c

Integrin alpha 3, also known as CD49c and VLA-3 alpha, is a type I membrane protein that heterodimerizes with Integrin beta 1. alpha 3 beta 1 is a cell surface adhesion molecule that functions as a receptor for laminin, fibronectin, collagen, epiligrin, thrombospondin and chondroitin sulfate proteoglycan 4. The extracellular domain of human and mouse Integrin alpha 3 share approximately 87% amino acid sequence homology.

Entrez Gene IDs

3675 (Human); 16400 (Mouse)

Alternate Names

antigen identified by monoclonal J143; CD49 antigen-like family member C; CD49c antigen; CD49c; FLJ34631; FLJ34704; FRP-2; Galactoprotein B3; GAP-B3; GAPB3CD49C; Integrin alpha 3; integrin alpha-3; integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor); ITGA3; MSK18; VCA-2; very late activation protein 3 receptor, alpha-3 subunit; VL3A; VLA-3 subunit alpha; VLA3a