Mouse Integrin alpha 5/CD49e Antibody

Name: Mouse Integrin alpha 5/CD49e Antibody
Brand: R&D Systems
SKU: MAB1754
Price: 429 USD
Availability: InStock

R&D Systems | Catalog # MAB1754

Submit a review

Citations (1)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 235112

View all Integrin alpha 5/CD49e Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e
Phe45-Asn999 (Gln68Glu)
Accession # P11688

Specificity

Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Mouse Integrin alpha 5/CD49e Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: B16‑F1 mouse melanoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed L-929 mouse fibroblast cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Integrin alpha 5/CD49e

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin alpha 5/CD49e

Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).