Mouse Integrin  alpha 5/CD49e Antibody

Catalog # Availability Size / Price Qty
MAB1754
MAB1754-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse Integrin  alpha 5/CD49e Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 5/CD49e in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 235112
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Integrin alpha 5/CD49e
Phe45-Asn999 (Gln68Glu)
Accession # P11688
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Integrin  alpha 5/CD49e
Flow Cytometry
2.5 µg/106 cells
B16‑F1 mouse melanoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
Immersion fixed L-929 mouse fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It is expressed on thymocytes, mast cells, activated T lymphocytes, and some mouse cell lines. It functions as a receptor for fibronectin (1‑4).

References
  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Kinashi, T. and T.A. Springer (1994) Blood Cells 20:25.
  3. Halvorson, M.J. et al. (1995) J. Immunol. 55:4567.
  4. Uhlenkott, E.C. et al. (1996) Clin. Exp. Metastasis 14:125.
Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Citation for Mouse Integrin  alpha 5/CD49e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. 14-3-3theta facilitates plasma membrane delivery and function of mechanosensitive connexin 43 hemichannels.
    Authors: Batra N, Riquelme M, Burra S, Jiang J
    J Cell Sci, 2013-10-25;127(0):137-46.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation, Western Blot

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