Mouse Integrin  alpha 8 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF4076
Integrin  alpha 8 in 4T1 Mouse Cell Line.
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Product Details
Citations (3)
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Mouse Integrin  alpha 8 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin alpha 8 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Integrin alpha 5 and recombinant mouse (rm) Integrin alpha 5 is observed, and less than 1% cross-reactivity with rmIntegrin alpha V is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8
Phe38-Phe1007
Accession # A2ARA8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
Mouse lung and rat lung
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Integrin a8 antibody in 4T1 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 8 in 4T1 Mouse Cell Line. Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse Integrin a8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot View Larger

Detection of Mouse Integrin  alpha 8 by Western Blot. Western blot shows lysates of Mouse Lung and Rat Lung. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Integrin  alpha 8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) followed by Streptavidin-HRP (Catalog # DY998) in 3% BSA. A specific band was detected for Integrin  alpha 8 at approximately 155 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Mesangial cells and not podocytes display considerable transient damage in the course of the EC model.Mice were analyzed at baseline (day 0), day 1, and day 7 following disease induction. A. Quantification of glomerular mesangial injury by alpha 8-integrin (mesangial cell marker) staining of kidney slices. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. **p<0.01, ***p<0.001 by one-way ANOVA; B. Representative alpha 8-integrin staining images in the course of the EC model. Scale bars correspond to 25 μm; C. Quantification of podocyte depletion and injury on kidney slices by WT1 and nephrin staining, respectively. Data are presented as mean ± SEM, n = 5/5/10 for day 0 /day 1 /day 7, respectively. *p<0.05, **p<0.01, ns- not significant by one-way ANOVA; D. Representative WT1/nephrin co-staining images in the course of the EC model. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse Integrin alpha 8 by Immunocytochemistry/Immunofluorescence RLCs selectively differentiate to intraglomerular mesangial cells during EC model.A. Representative confocal microscopy images for day 0 and day 7 of beta -gal and the EC markers ERG (upper panels) or CD31 (lower panels) co-stained kidney slices; B. Representative confocal microscopy images for day 0 and day 7 of beta -gal and the mesangial cell markers PDGF beta R (upper panels) or alpha 8-integrin (lower panels) co-stained kidney slices; C. Representative confocal microscopy images for day 0 and day 7 of beta -gal/WT1 (podocyte marker) co-stained kidney slices. 4′,6-diamidino-2-phenylindole (DAPI) was used as a nuclear marker throughout. The channels for green ( beta -gal) and red (corresponding cell marker) fluorescent signals in the dashed squares on day 7 are separately shown in the small right panels. Scale bars correspond to 25 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29771991), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse Integrin alpha 8 by Flow Cytometry View Larger

Detection of Mouse Integrin alpha 8 by Flow Cytometry Vascular development is limited in the reconstituted kidney organoids in vitro (A) Scheme for kidney organoid reconstitution and culture in vitro. Three types of kidney progenitors at E11.5 (NPs, SPs, and Hoxb7-GFP+ UBs) were aggregated overnight with or without ECs. The organoids were subsequently cultured at the air-liquid interface for 2–6 days (days 3–7). (B) Sorting of ECs, NP, and SPs. CD31+ and/or Flk1+ cells were sorted as ECs (left), and the cells in the CD31−/Flk1− fraction (left) were further sorted into Itga8+ NP and Pdgfra+ SP fractions (right). (C–F) Whole-mount staining of kidney organoids aggregated with ECs and cultured for the indicated days. GFP+ UB branching and CD31+ vasculature formation (C,D), as well as Nephrin+ glomerulus formation (E), were observed. No Cx40+ arterioles (F) were observed. (G) Section staining of glomeruli (nephrin). No CD31+ ECs were detected in glomeruli. (H–L) Whole-mount (H–K) or section (L) staining of kidney organoids aggregated without ECs and cultured for the indicated days. No apparent differences between the organoids with (C–G) and without (H–L) ECs were observed. Scale bars: 100 µm (C–F, H–K); 10 µm (G,L). Representative images of three organoids each with and without ECs from three independent experiments are shown. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30718617), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 8

Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin  alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin  alpha 8 protein, respectively.

Entrez Gene IDs
8516 (Human); 241226 (Mouse); 84381 (Rat)
Alternate Names
Integrin alpha 8; integrin alpha-8; integrin, alpha 8; ITGA8

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Citations for Mouse Integrin  alpha 8 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis
    Authors: AE Jarmas, EW Brunskill, P Chaturvedi, N Salomonis, R Kopan
    Nature Communications, 2021-11-03;12(1):6332.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Authors: Y Murakami, H Naganuma, S Tanigawa, T Fujimori, M Eto, R Nishinakam
    Sci Rep, 2019-02-04;9(1):1172.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Flow Cytometry
  3. Regenerative potential of induced pluripotent stem cells derived from patients undergoing haemodialysis in kidney regeneration
    Authors: S Tajiri, S Yamanaka, T Fujimoto, K Matsumoto, A Taguchi, R Nishinakam, HJ Okano, T Yokoo
    Sci Rep, 2018-10-08;8(1):14919.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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