Detects mouse Integrin alpha 8 in Western blots. In Western blots, less than 5% cross-reactivity with recombinant human Integrin alpha 5 and recombinant mouse (rm) Integrin alpha 5 is observed, and less than 1% cross-reactivity with rmIntegrin alpha V is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8 Phe38-Phe1007 Accession # A2ARA8
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Recombinant Mouse Integrin alpha 8
2.5 µg/106 cells
4T1 mouse breast cancer cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Integrin alpha 8 in 4T1 Mouse Cell Line. Integrin alpha 8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse Integrin alpha 8 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha 8 protein, respectively.
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