Mouse/Rat Integrin  alpha 8 Antibody

(5 citations)
(3 Reviews)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Integrin alpha 8 in Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Integrin  alpha 8 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5, recombinant mouse (rm) Integrin alpha 5, rhIntegrin alpha V and rmIntegrin  alpha V is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8
    Phe38-Phe1007
    Accession # A28ARA8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    4T1 mouse breast cancer cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Integrin  alpha 8 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin  alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin  alpha 8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Integrin  alpha 8 in 4T1 Mouse Breast Cancer Cell Line. Integrin  alpha 8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin  alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse and Rat Integrin  alpha 8 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha 8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin  alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin  alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin  alpha 8 protein, respectively.
  • Entrez Gene IDs:
    8516 (Human); 241226 (Mouse); 84381 (Rat)
  • Alternate Names:
    Integrin alpha 8; integrin alpha-8; integrin, alpha 8; ITGA8
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage
    PLoS ONE, 2016;11(12):e0168343.
    Species: Canine
    Sample Type: Whole Tissue
    Application: IHC
  3. Laminin alpha2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis.
    Authors: Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, Cosgrove D
    PLoS ONE, 2014;9(6):e99083.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013;504(7479):277-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  5. Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins.
    J. Cell Biol., 2012;197(5):677-89.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Cell and Tissue Staining Kits
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 3 reviews)

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Read what people are saying who have used Mouse/Rat Integrin  alpha 8 Antibody.

We have 2 reviews tested in 1 species: Mouse.
We have 3 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Mouse/Rat Integrin  alpha 8 Antibody AF4076
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Excellent
 ICC/IF Mouse Anonymous 08/10/2017
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Image Details
Immunocytochemistry/Immunofluorescence Mouse/Rat Integrin  alpha 8 Antibody AF4076
Immunocytochemistry/Immunofluorescence: Mouse/Rat Integrin  alpha 8 Antibody [AF4076]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedEmbryonic kidney
SpeciesMouse
Immunocytochemistry/Immunofluorescence Mouse/Rat Integrin  alpha 8 Antibody AF4076
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Excellent
 ICC/IF Mouse Anonymous 05/13/2016
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Image Details
Immunocytochemistry/Immunofluorescence Mouse/Rat Integrin  alpha 8 Antibody AF4076
Immunocytochemistry/Immunofluorescence: Mouse/Rat Integrin  alpha 8 Antibody [AF4076]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedKidney tissue
SpeciesMouse
Western Blot Mouse Integrin  alpha 8 Antibody AF4076
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Excellent
 WB Anonymous 02/22/2016
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Image Details
Western Blot Mouse Integrin  alpha 8 Antibody AF4076
Western Blot: Mouse Integrin  alpha 8 Antibody [AF4076]

Summary

ApplicationWestern Blot
Sample TestedStable HEK293 cells expressing full length ITGA8

Other Experimental Details

Other Experimental DetailsStables HEK293 expressing ITGA8 were lysed in RIPA and used for western blot analysis. Blot was incubated overnight with 5% milk blocking solution followed by an overnight incubation with 0.4ug/ml of goat anti-ITGA8 in 5% milk. Blot was incubated the next day with 1:3,000 anti-goat HRP secondary antibody and developed employing a film processor. 293 ITGA8: Stable cell line. 293: untransfected cells. Actin was used as loading control.

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