Mouse/Rat Integrin  alpha 8 Antibody

Catalog # Availability Size / Price Qty
AF4076
AF4076-SP
Detection of Mouse Integrin  alpha 8 by Western Blot.
6 Images
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (3)

Mouse/Rat Integrin  alpha 8 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Integrin alpha 8 in Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Integrin  alpha 8 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5, recombinant mouse (rm) Integrin alpha 5, rhIntegrin alpha V and rmIntegrin  alpha V is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8
Phe38-Phe1007
Accession # A28ARA8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Integrin a8 antibody by Western Blot. View Larger

Detection of Mouse Integrin alpha 8 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Integrin a8 antibody in 4T1 Mouse Breast Cancer Cell Line by Immunocytochemistry (ICC). View Larger

Integrin alpha 8 in 4T1 Mouse Breast Cancer Cell Line. Integrin a8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse and Rat Integrin a8 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse and Rat Integrin alpha 8 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin a8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin a8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Canine Integrin alpha 8 by Immunocytochemistry/Immunofluorescence Integrin alpha 8 co-localizes with laminin 211 in the GBM of AS but not WT dogs.A-C: Dual immunofluorescence immunostaining of kidney from a WT dog; 63x 1.4 n.a. oil. The GBM was localized with anti-collagen alpha 5 ( alpha 5(IV)) and the mesangium was localized with anti-integrin alpha 8 (INT alpha 8). D-I: Dual immunofluorescence immunostaining of kidney tissue from an AS dog at milestone 2. Laminin 211, produced by mesangial cells, was labeled with anti-laminin alpha 2 (LAMA2) and mesangial cells were localized with anti-integrin alpha 8 (INT alpha 8), demonstrating co-localization of laminin 211 with mesangial cell extension in capillary loops. Images D-F were taken with 40x1.3 n.a. oil; images G-I were taken with 63x1.4 n.a. oil with 2X zoom. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0168343), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 8

Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin  alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin  alpha 8 protein, respectively.

Entrez Gene IDs
8516 (Human); 241226 (Mouse); 84381 (Rat)
Alternate Names
Integrin alpha 8; integrin alpha-8; integrin, alpha 8; ITGA8

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Citations for Mouse/Rat Integrin  alpha 8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Progenitor-derived endothelin controls dermal sheath contraction for hair follicle regression
    Authors: P Martino, R Sunkara, N Heitman, M Rangl, A Brown, N Saxena, L Grisanti, D Kohan, M Yanagisawa, M Rendl
    Nature Cell Biology, 2023-01-30;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  2. Distinct Regulatory Programs Control the Latent Regenerative Potential of Dermal Fibroblasts during Wound Healing
    Authors: S Abbasi, S Sinha, E Labit, NL Rosin, G Yoon, W Rahmani, A Jaffer, N Sharma, A Hagner, P Shah, R Arora, J Yoon, A Islam, A Uchida, CK Chang, JA Stratton, RW Scott, FMV Rossi, TM Underhill, J Biernaskie
    Cell Stem Cell, 2020-08-04;27(3):396-412.e6.
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  3. Aberrant DNA methylation of Tgfb1 in diabetic kidney mesangial cells
    Authors: S Oba, N Ayuzawa, M Nishimoto, W Kawarazaki, K Ueda, D Hirohama, F Kawakami-M, T Shimosawa, T Marumo, T Fujita
    Sci Rep, 2018-11-05;8(1):16338.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Role of integrin alpha8 in murine model of lung fibrosis
    Authors: CF Hung, CL Wilson, YH Chow, LM Schnapp
    PLoS ONE, 2018-05-29;13(5):e0197937.
    Species: Mouse
    Sample Types: Protein, Whole Tissue
    Applications: IHC, Western Blot
  5. Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development
    Authors: N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi
    Dev. Cell, 2017-05-22;41(4):349-365.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Activation of Hypoxia Signaling in Stromal Progenitors Impairs Kidney Development
    Authors: K Gerl, D Steppan, M Fuchs, C Wagner, C Willam, A Kurtz, B Kurt
    Am. J. Pathol., 2017-05-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017-02-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  8. X-Linked Alport Dogs Demonstrate Mesangial Filopodial Invasion of the Capillary Tuft as an Early Event in Glomerular Damage
    PLoS ONE, 2016-12-13;11(12):e0168343.
    Species: Canine
    Sample Types: Whole Tissue
    Applications: IHC
  9. Laminin alpha2-mediated focal adhesion kinase activation triggers Alport glomerular pathogenesis.
    Authors: Delimont D, Dufek B, Meehan D, Zallocchi M, Gratton M, Phillips G, Cosgrove D
    PLoS ONE, 2014-06-10;9(6):e99083.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. Distinct fibroblast lineages determine dermal architecture in skin development and repair.
    Authors: Driskell R, Lichtenberger B, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron S, Herault Y, Pavlovic G, Ferguson-Smith A, Watt F
    Nature, 2013-12-12;504(7479):277-81.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Basement membrane assembly of the integrin alpha8beta1 ligand nephronectin requires Fraser syndrome-associated proteins.
    J. Cell Biol., 2012-05-21;197(5):677-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Reviews for Mouse/Rat Integrin  alpha 8 Antibody

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Mouse/Rat Integrin  alpha 8 Antibody
By Anonymous on 08/10/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Embryonic kidney Species: Mouse

Mouse/Rat Integrin  alpha 8 Antibody
By Anonymous on 05/13/2016
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Kidney tissue Species: Mouse

Mouse Integrin  alpha 8 Antibody
By Anonymous on 02/22/2016
Application: WB Sample Tested: Stable HEK293 cells expressing full length ITGA8 Species: input species here, HEK293 cells, Mouse

Stables HEK293 expressing ITGA8 were lysed in RIPA and used for western blot analysis. Blot was incubated overnight with 5% milk blocking solution followed by an overnight incubation with 0.4ug/ml of goat anti-ITGA8 in 5% milk. Blot was incubated the next day with 1:3,000 anti-goat HRP secondary antibody and developed employing a film processor. 293 ITGA8: Stable cell line. 293: untransfected cells. Actin was used as loading control.