Detects mouse and rat Integrin alpha 8 in Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant human (rh) Integrin alpha 8 is observed, and less than 1% cross-reactivity with rhIntegrin alpha 5, recombinant mouse (rm) Integrin alpha 5, rhIntegrin alpha V and rmIntegrin alpha V is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin alpha 8 Phe38-Phe1007 Accession # A28ARA8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Mouse Integrin alpha 8 by Western Blot.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Integrin alpha 8 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Integrin alpha 8 in 4T1 Mouse Breast Cancer Cell Line.
Integrin alpha 8 was detected in immersion fixed 4T1 mouse breast cancer cell line using Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Mouse and Rat Integrin alpha 8 by Simple WesternTM.
Simple Western lane view shows lysates of mouse lung tissue and rat lung tissue, loaded at 0.2 mg/mL. A specific band was detected for Integrin alpha 8 at approximately 150 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat Integrin alpha 8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4076) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin alpha 8
Integrin alpha 8 is a 170‑200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. alpha 8 beta 1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGF-beta 1 and nephronectin. Mouse Integrin alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 969 aa extracellular region (aa 38‑1006) and a 29 aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin alpha 8 shares 96% and 90% aa sequence identity with rat and human Integrin alpha 8 protein, respectively.
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We have 3 reviews tested in 2 species: input species here,HEK293 cells,Mouse, Mouse.
We have 3 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Western Blot.
Western Blot: Mouse Integrin alpha 8 Antibody [AF4076].
Stable HEK293 cells expressing full length ITGA8
input species here,HEK293 cells,Mouse
Other Experimental Details
Other Experimental Details
Stables HEK293 expressing ITGA8 were lysed in RIPA and used for western blot analysis. Blot was incubated overnight with 5% milk blocking solution followed by an overnight incubation with 0.4ug/ml of goat anti-ITGA8 in 5% milk. Blot was incubated the next day with 1:3,000 anti-goat HRP secondary antibody and developed employing a film processor. 293 ITGA8: Stable cell line. 293: untransfected cells. Actin was used as loading control.