Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 288422
View all Kininogen Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Kininogen
Glu21-Ser661
Accession # O08677

Specificity

Detects mouse Kininogen in direct ELISAs and Western blots. In Western blots, reacts with the full length mouse Kininogen (aa 21‑661), but not with the light chain alone. In Western blots, no cross-reactivity with recombinant human (rh) Cystatin D, F, SA, SN, recombinant mouse (rm) Cystatin A, B, C, E/M, rmCystatin/Stefin Homolog, rmFetuin A, rhFetuin B, rmHPRG, rhKininogen, or rhKininostatin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Kininogen Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Kininogen High Molecular Weight (HKa) (Catalog # 2206-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Mouse Kininogen High Molecular Weight (HKa) (Catalog # 2206-PI)
under non-reducing conditions only

Reviewed Applications

Read 1 review rated 5 using MAB2206 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Kininogen

Kininogen, also known as alpha 2-thiol proteinase inhibitor, is a multi-function protein. There are two alternatively spliced forms, designated as the high molecular weight (HMW) and low MW (LMW) forms (1). The mouse HMW form is synthesized as a 661 amino acid (aa) precursor with a signal peptide (aa 1‑20). The mature chain (aa 21‑661) is further processed into the heavy (aa 21‑379) and the light (aa 389‑661) chains. The active peptide bradykinin (aa 380‑388) is released which has a variety of functions including muscle contraction, hypotension and inflammation. The heavy chain consists of three cystatin-like domains which are responsible for inhibiting cysteine proteases. The light chain consists of a His‑rich domain which is associated with the clotting activity. In comparison to the HMW form, the LMW Kininogen (432 residues) has the same sequence in its heavy chain and bradykinin but has a different sequence in its light chain (aa 401‑432). The LMW form is not involved in blood clotting.

References

  1. Takano, M. et al. (1997) Biochim. Biophys. Acta 1352:222.

Alternate Names

alpha 2-thiol Proteinase Inhibitor, BDK, KNG1

Entrez Gene IDs

3827 (Human); 16644 (Mouse); 24903 (Rat)

Gene Symbol

KNG1

UniProt

O08677

Additional Kininogen Products

Product Documents for Mouse Kininogen Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Kininogen Antibody

For research use only

Customer Reviews for Mouse Kininogen Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse Kininogen Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Mouse Kininogen Antibody
    Name: Anonymous
    Application: Functional Assay
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 10/12/2019
    Mouse Kininogen Antibody MAB2206

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail