Mouse L1CAM Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5674R-100UG
Mouse L1CAM Alexa Fluor® 647-conjugated Antibody in Flow Cytometry
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Mouse L1CAM Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm)MAdCAM-1, or rmOCAM is observed.
Source
Monoclonal Rat IgG2A Clone # 555
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse cerebellum-derived partially purified L1CAM
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of L1CAM in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of L1CAM in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse L1CAM Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB5674R, filled histogram) or isotype control antibody (Catalog # IC006R, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: L1CAM

L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein of the immunoglobulin superfamily and L1/neurofascin/NgCAM family (1, 2). L1CAM is expressed by neurons, especially by growing axons on their growth cones (2). Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM (2). Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail (3). Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination (4). L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins (4). L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub (4). The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration (4). L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis (1). Overexpression promotes tumor cell invasion and motility, growth in nude mice, and tumor metastasis (1). Proteolytic processing by ADAM10 and
presenilin/ gamma -secretase is essential for the nuclear signaling of L1CAM in human carcinoma cell lines (1). Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome (5, 6).

References
  1. Riedle, S. et al. (2009) Biochem. J. 420:391.
  2. Kenwrick, S. and P. Doherty (1998) Bioessays 20:668.
  3. Crossin, K.L. and L.A. Krushel (2000) Dev. Dyn. 218:260.
  4. Maretzky, T. et al. (2005) Mol. Cell. Biol. 25:9040.
  5. Kamiguchi, H. et al. (1998) Mol. Cell Neurosci. 12:48.
  6. Striha, L. et al. (2000) J. Child Neurol. 15:239.
Long Name
Cell Adhesion Molecule L1
Entrez Gene IDs
3897 (Human); 16728 (Mouse); 50687 (Rat)
Alternate Names
antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; L1CAM; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1

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