Mouse L1CAM PE-conjugated Antibody

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Detection of L1CAM in Mouse Splenocytes by Flow Cytometry.
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Mouse L1CAM PE-conjugated Antibody Summary

Species Reactivity
Detects mouse L1CAM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ALCAM, rhBCAM, rhEPCAM, rhMCAM, rhNCAM, rhNCAM-L1, rhOBCAM, recombinant mouse (rm) MAdCAM-1, or rmOCAM is observed.
Monoclonal Rat IgG2A Clone # 555
Protein A or G purified from hybridoma culture supernatant
Mouse cerebellum-derived partially purified L1CAM
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes and Neuro‑2A mouse neuroblastoma cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of L1CAM antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of L1CAM in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse L1CAM PE-conjugated Monoclonal Antibody (Catalog # FAB5674P, filled histogram) or isotype control antibody (IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of L1CAM in Neuro-2A cells by Flow Cytometry. Neuro-2A cells were stained with Rat Anti-Mouse L1CAM PE‑conjugated Monoclonal Antibody (Catalog # FAB5674P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: L1CAM

L1CAM, also known as Neural Cell Adhesion Molecule L1 (NCAM-L1) and CD171, is a 200‑220 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily, L1/Neurofascin/NgCAM family of molecules. L1CAM is expressed by neurons, especially by growing axons on their growth cones. Non-neuronal cells such as Schwann cells, astrocytes, epithelial cells, and cells of myelomonocytic and lymphoid origin also express L1CAM. Mature mouse L1CAM consists of a 1104 amino acid (aa) extracellular domain (ECD) with 6 Ig-like domains and 5 fibronectin type-III domains, a 23 aa transmembrane region, and a 114 aa cytoplasmic tail. Mouse L1CAM shares 88% aa sequence identity with human L1CAM. L1CAM is critical for neural development. Specifically, L1CAM plays a key role in neuronal cell migration, axon outgrowth, axon fasciculation, synaptogenesis, and myelination. L1CAM mediates homophilic cell-cell interaction but also binds heterophilically with Axonin-1, CD24, CD9, Neurocan and several Integrins. L1CAM can undergo membrane‑proximal cleavage by ADAM10 and ADAM17, leading to the release of the soluble ECD and the generation of a membrane‑bound stub. The soluble ECD can serve as a substrate for integrin-mediated cell adhesion, thereby stimulating cellular motility and cell migration. L1CAM also plays a role in the ontogeny of human tumors, and its expression is linked to poor prognosis. Proteolytic processing by ADAM10 and presenilin/ gamma -secretase is essential for "forward signaling" where an L1CAM intracellular domain translocates to the nucleus and participates in gene regulation. Defects in L1CAM are the cause of the neurological MASA/CRASH syndrome. In addition, uncleaved L1CAM can cluster with Integrin alpha 5 either in-cis, or in-trans, inducing IL-1 beta expression and subsequent NF- kappa B activation. This is referred to as "reverse signaling".

Long Name
Cell Adhesion Molecule L1
Entrez Gene IDs
3897 (Human); 16728 (Mouse); 50687 (Rat)
Alternate Names
antigen identified by monoclonal R1; CAML1; CAML1N-CAML1; CD171 antigen; CD171; HSAS; HSAS1; L1 cell adhesion molecule; L1CAM; MASA; MIC5; N-CAM-L1; NCAM-L1; neural cell adhesion molecule L1; S10; SPG1

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Mouse L1CAM PE-conjugated Antibody
By Christian Ostalecki on 06/08/2018
Application: ICC/IF Sample Tested: Colon cancer tissue Species: Mouse