Mouse LIF R alpha PE-conjugated Antibody

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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LIF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LIF R alpha is observed.
  • Source
    Monoclonal Rat IgG1 Clone # 673602
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse LIF R alpha
    Leu44-Ser828
    Accession # P42703
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF R alpha PE‑conjugated Monoclonal Antibody (Catalog # FAB5990P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LIF R alpha
Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 185-190 kDa type I transmembrane protein that belongs to the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M. Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain. Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD. LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity. Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130. The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M. gp130 and LIF R alpha  also associate with different ligand-specific receptors to form signaling receptor complexes for other IL-6 family ligands such as CNTF, CT-1 and CLC. The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha. LIF R alpha is widely expressed, and cells reported to contain LIF R alpha include hepatic sinusoidal endothelium, steroidal adrenal cortical cells, uterine luminal columnar epithelium, cardiac muscle cells, embryonic stem cells, odontoblasts, forebrain neural precursors, fibroblasts (3T3), osteoblasts, megakaryocytes, osteocytes, activated macrophages and sympathetic neurons. LIF induces the proliferation, differentiation, and activation of cells in many tissues. In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus.
  • Long Name:
    Leukemia Inhibitory Factor Receptor alpha
  • Entrez Gene IDs:
    3977 (Human); 16880 (Mouse); 81680 (Rat)
  • Alternate Names:
    CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG1 PE-conjugated Antibody

Ctrl IC005P 1  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Mouse LIF R alpha  PE-conjugated Antibody FAB5990P Array FAB5990P
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 Flow Mouse Anonymous 04/05/2018
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Flow Cytometry Mouse LIF R alpha  PE-conjugated Antibody FAB5990P
Flow Cytometry: Mouse LIF R alpha PE-conjugated Antibody [FAB5990P].

Summary

Application Flow Cytometry
Sample Tested Mouse spleenocytes
Species Mouse

Other Experimental Details

Other Experimental Details Spleenocytes from C57BL/6 mouse were gated on Live, CD11b-, CD3-,CD19-, NK1.1- and Ly6G-. The matched isotype control is show in gray and LIFR PE in blue.

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