Mouse LIFR alpha Alexa Fluor® 594-conjugated Antibody

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FAB5990T-100UG

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Mouse LIFR alpha Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LIF R alpha is observed.
Source
Monoclonal Rat IgG1 Clone # 673602
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LIF R alpha
Leu44-Ser828
Accession # P42703
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
D3 mouse embryonic stem cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain (2, 3). Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD (4). LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity (5). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (6, 7). The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (6). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (8, 9). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (10, 11). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (4, 12-14).

References
  1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
  2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
  3. Tomida, M. et al. (1994) J. Biochem. 115:557.
  4. Owczarek, C.M. et al. (1996) J. Biol. Chem. 271:5495.
  5. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
  6. Gearing, D.P. et al. (1992) Science 255:1434.
  7. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
  8. Ip, N.Y. et al. (1992) Cell 69:1121.
  9. Davis, S. et al. (1993) Science 260:1805.
  10. Metcalf, D (2003) Stem Cells 21:5.
  11. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
  12. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  13. Stewart, C.L. et al. (1992) Nature 359:76.
  14. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.
Long Name
Leukemia Inhibitory Factor Receptor alpha
Entrez Gene IDs
3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)
Alternate Names
CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR alpha; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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