Mouse LIFR alpha PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5990P
Detection of LIF R alpha  in D3 Mouse Cell Line by Flow Cytometry.
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Mouse LIFR alpha PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LIF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LIF R alpha is observed.
Source
Monoclonal Rat IgG1 Clone # 673602
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LIF R alpha
Leu44-Ser828
Accession # P42703
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of LIF Ra antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of LIF R alpha in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse LIF Ra PE-conjugated Monoclonal Antibody (Catalog # FAB5990P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha ), also known as LIFR beta and CD118, is a 185-190 kDa type I transmembrane protein that belongs to the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M. Mature mouse LIF R alpha consists of a 785 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 25 aa transmembrane segment and a 239 aa cytoplasmic domain. Within the ECD, mouse LIF R alpha shares 73% and 90% aa sequence identity with human and rat LIF R alpha, respectively. Alternative splicing generates a 90 kDa soluble form of the mouse LIF R alpha ECD. LIF R alpha binds the pleiotropic cytokine LIF with low affinity, and the soluble isoform retains LIF-binding activity. Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130. The LIF R alpha /gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M. gp130 and LIF R alpha  also associate with different ligand-specific receptors to form signaling receptor complexes for other IL-6 family ligands such as CNTF, CT-1 and CLC. The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha. LIF R alpha is widely expressed, and cells reported to contain LIF R alpha include hepatic sinusoidal endothelium, steroidal adrenal cortical cells, uterine luminal columnar epithelium, cardiac muscle cells, embryonic stem cells, odontoblasts, forebrain neural precursors, fibroblasts (3T3), osteoblasts, megakaryocytes, osteocytes, activated macrophages and sympathetic neurons. LIF induces the proliferation, differentiation, and activation of cells in many tissues. In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus.

Long Name
Leukemia Inhibitory Factor Receptor alpha
Entrez Gene IDs
3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)
Alternate Names
CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR alpha; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS

Product Datasheets

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Mouse LIF R alpha PE-conjugated Antibody
By Anonymous on 04/05/2018
Application: Flow Sample Tested: Mouse spleenocytes Species: Mouse

Spleenocytes from C57BL/6 mouse were gated on Live, CD11b-, CD3-,CD19-, NK1.1- and Ly6G-. The matched isotype control is show in gray and LIFR PE in blue.