Mouse LIX Alexa Fluor™ Plus 680‑conjugated Antibody

R&D Systems | Catalog # AF433AFP680

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Neutralization

Label

Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)

Antibody Source

Polyclonal Goat IgG
View all LIX Primary Antibodies »

Product Specifications

Specificity

Detects mouse LIX in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human GCP‑2, recombinant rat (rr) CINC‑2 alpha, rrCINC-2 beta, recombinant mouse (rm) KC, and rmMIP‑2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse LIX Alexa Fluor™ Plus 680‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Neutralization

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LIX

LIX ( Liposaccharide-Induced CXC chemokine; also GARG-8 and Cxcl5) is a secreted 8-9 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemokinetic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Mouse LIX is synthesized as a 132 amino acid (aa) precursor that contains a 40 aa signal sequence, a 78 aa mature region (aa 41-118), and a cleavable 14 aa C-terminus. The mature region possesses an ELR/GluLeuArg motif between aa 50-52, and an alpha -family characteristic CxC motif between aa 53-55. Although there are no known splice variants of mouse LIX, considerable proteolytic processing occurs at both the N- and C-termini over aa 41-132. This may reduce the MW in SDS-PAGE by as much as 3 kDa. The majority of LIX appears to start between aa 47-50, and this is positively correlated with bioactivity. Over aa 41-118, mouse LIX shares 73% aa sequence identity with rat LIX. Although not a strict ortholog, mouse LIX shares 63% aa sequence identity with human GCP-2.

Alternate Names

LIX

Entrez Gene IDs

20311 (Mouse); 60665 (Rat)

Gene Symbol

CXCL5

UniProt

P50228

Additional LIX Products

Product Documents for Mouse LIX Alexa Fluor™ Plus 680‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse LIX Alexa Fluor™ Plus 680‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Chemokines and Receptors

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