LPS-induced CXC chemokine (LIX) is a member of the murine chemokine family. It is 132 amino acids (aa) in length with a predicted molecular weight of 14 kDa prior to cleavage of the N-terminal signal peptide (aa 1–40). LIX has no true human ortholog but is closely related to human CXCL5/ENA-78 and CXCL6/GCP-2. It is widely expressed, and is secreted by multiple cell types including fibroblasts, thymic epithelium, platelets, vascular endothelium, and hepatocytes. LIX acts as a proinflammatory chemokine and is chemotactic for neutrophils.
Mouse LIX Quantikine ELISA Kit
R&D Systems | Catalog # MX000
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (13 µL), Platelet-poor Heparin Plasma (13 µL), Urine (13 µL)
Sensitivity
1.74 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
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Product Summary for Mouse LIX Quantikine ELISA Kit
The Quantikine Mouse CXCL5/LIX Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse LIX levels in cell culture supernates, tissue lysates, serum, platelet-poor plasma, and urine. It contains recombinant mouse LIX and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse LIX accurately. Results obtained using natural mouse LIX showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse LIX.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse LIX
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Platelet-poor Heparin Plasma, Serum, Serum, Tissue Lysates, Tissue Lysates, Urine, Urine
| Intra-Assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| n | 20 | 20 | 20 |
| Mean (pg/mL) | 42.7 | 133 | 407 |
| Standard Deviation | 1.72 | 5.93 | 10.9 |
| CV% | 4.0 | 4.5 | 2.7 |
Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Tissue Lysates, Urine
| Inter-Assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| n | 20 | 20 | 20 |
| Mean | 41.8 | 120 | 393 |
| Standard Deviation | 2.81 | 4.64 | 20.3 |
| CV% | 6.7 | 3.9 | 5.2 |
Recovery for Mouse LIX Quantikine ELISA Kit
The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 114 | 105-120 |
| Platelet-poor EDTA Plasma (n=4) | 100 | 92-109 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 85-99 |
| Serum (n=4) | 87 | 82-94 |
| Tissue Lysates (n=4) | 100 | 87-113 |
| Urine (n=4) | 91 | 84-96 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse LIX were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
Scientific Data Images for Mouse LIX Quantikine ELISA Kit
Mouse LIX ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LIX
Additional LIX Products
Product Documents for Mouse LIX Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse LIX Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Mouse LIX Quantikine ELISA Kit
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Protocols
View specific protocols for Mouse LIX Quantikine ELISA Kit (MX000):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
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