Mouse LIX Quantikine ELISA Kit Summary
Cell Culture Supernates, Tissue Homogenates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine
|Intra-Assay Precision||Inter-Assay Precision|
The recovery of mouse LIX spiked to three levels throughout the range of the assay was evaluated.
|Sample Type||Average % Recovery||Range %|
|Cell Culture Samples (n=4)||114||105-120|
|Platelet-poor EDTA Plasma (n=4)||100||92-109|
|Platelet-poor Heparin Plasma (n=4)||91||85-99|
|Tissue Homogenates (n=4)||100||87-113|
Mouse LIX ELISA Standard Curve
Preparation and Storage
LPS-induced CXC chemokine (LIX) is a member of the murine chemokine family. It is 132 amino acids (aa) in length with a predicted molecular weight of 14 kDa prior to cleavage of the N-terminal signal peptide (aa 1–40). LIX has no true human ortholog but is closely related to human CXCL5/ENA-78 and CXCL6/GCP-2. It is widely expressed, and is secreted by multiple cell types including fibroblasts, thymic epithelium, platelets, vascular endothelium, and hepatocytes. LIX acts as a proinflammatory chemokine and is chemotactic for neutrophils.
Assay ProcedureRefer to the product
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse LIX Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 6
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Protective Effect of Traditional Chinese Medicine Formula RP on Lung Microenvironment in Pre-Metastasis Stage of Breast Cancer
Authors: Y Ye, L Pei, C Wu, S Liu
Integr Cancer Ther, 2019;18(0):1534735419876.
Sample Types: BALF
Snail promotes ovarian cancer progression by recruiting myeloid-derived suppressor cells via CXCR2 ligand upregulation
Authors: M Taki, K Abiko, T Baba, J Hamanishi, K Yamaguchi, R Murakami, K Yamanoi, N Horikawa, Y Hosoe, E Nakamura, A Sugiyama, M Mandai, I Konishi, N Matsumura
Nat Commun, 2018;9(1):1685.
Sample Types: Serum
Sitagliptin, a dipeptidyl peptidase-4 inhibitor, suppresses CXCL5 and SDF-1 and does not accelerate intestinal neoplasia formation in Apc(Min/+) mice fed a high-fat diet
Authors: K Fujiwara, T Inoue, Y Henmi, Y Hirata, Y Naka, A Hara, K Kakimoto, S Nouda, T Okada, K Kawakami, T Takeuchi, K Higuchi
Oncol Lett, 2017;14(4):4355-4360.
Sample Types: Plasma
Upregulation of SOCS3 in lung CD4+ T cells in a mouse model of chronic PA lung infection and suppression of Th17?mediated neutrophil recruitment in exogenous SOCS3 transfer in vitro
Authors: FM Ding, RM Liao, YQ Chen, GG Xie, PY Zhang, P Shao, M Zhang
Mol Med Rep, 2017;0(0):.
Sample Types: Cell Culture Supernates
Sharpin Controls Osteogenic Differentiation of Mesenchymal Bone Marrow Cells.
Authors: Jeschke A, Catala-Lehnen P, Sieber S, Bickert T, Schweizer M, Koehne T, Wintges K, Marshall R, Mautner A, Duchstein L, Otto B, Horst A, Amling M, Kreienkamp H, Schinke T
J Immunol, 2015;195(8):3675-84.
Sample Types: Serum
Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost., 2012;10(8):1616-23.
Sample Types: Plasma
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