Mouse LYVE-1 Alexa Fluor® 488-conjugated Antibody Summary
Accession # Q8BHC0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB2125G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.
LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).
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Citations for Mouse LYVE-1 Alexa Fluor® 488-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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High-resolution 3D imaging and topological mapping of the lymph node conduit system
Authors: ID Kelch, G Bogle, GB Sands, ARJ Phillips, IJ LeGrice, PR Dunbar
PLoS Biol., 2019;17(12):e3000486.
Sample Types: In Vivo
Applications: In Vivo
Repetitive ischemic injuries to the kidneys result in lymph node fibrosis and impaired healing
Authors: OH Maarouf, M Uehara, V Kasinath, Z Solhjou, N Banouni, B Bahmani, L Jiang, OA Yilmam, I Guleria, SB Lovitch, JL Grogan, P Fiorina, PT Sage, JS Bromberg, MM McGrath, R Abdi
JCI Insight, 2018;3(13):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic
Proc Natl Acad Sci USA, 2016;113(38):10643-10648.
Sample Types: Whole Tissue
Exposure to Ionizing Radiation Induces the Migration of Cutaneous Dendritic Cells by a CCR7-Dependent Mechanism.
Authors: Cummings, Ryan J, Gerber, Scott A, Judge, Jennifer, Ryan, Julie L, Pentland, Alice P, Lord, Edith M
J Immunol, 2012;189(9):4247-57.
Sample Types: Whole Tissue
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