Mouse LYVE-1 Antibody

Catalog # Availability Size / Price Qty
MAB2125-SP
MAB2125-500
MAB2125-100
Mouse LYVE-1 Antibody in Data
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Product Details
Citations (25)
FAQs
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Reviews (2)

Mouse LYVE-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 223322
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF/3 mouse pro-B cell line transfected with mouse LYVE-1
Ala24-Thr234
Accession # Q8BHC0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.02-0.1 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Mouse LYVE-1 (Catalog # 2125-LY) coated at 5 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse LYVE-1 antibody by Western Blot. View Larger

Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of LYVE-1 antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry LYVE-1 antibody in NS0 mouse myeloma cell line transfected with mouse LYVE-1 by Immunocytochemistry (ICC). View Larger

LYVE-1 in NS0 mouse myeloma cell line transfected with mouse LYVE-1. LYVE-1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse LYVE-1 (positive control) and wild type NS0 mouse myeloma cell line (negative control) using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry LYVE-1 antibody in Mouse Intestine by Immunohistochemistry (IHC-Fr). View Larger

LYVE-1 in Mouse Intestine. LYVE-1 was detected in immersion fixed frozen sections of mouse intestine tissue using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in lymphatic vessels. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

References
  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.
  7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.
  8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
Long Name
Lymphatic Vessel Endothelial Hyaluronan Receptor 1
Entrez Gene IDs
10894 (Human); 114332 (Mouse); 293186 (Rat)
Alternate Names
cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; UNQ230/PRO263; XLKD1

Product Datasheets

Citations for Mouse LYVE-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas
    Authors: X Jia, F Gábris, Ó Jacobsen, G Bedics, B Botz, Z Helyes, Z Kellermaye, D Vojkovics, G Berta, N Nagy, Z Jakus, P Balogh
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Single-Cell Analysis Reveals Heterogeneity of High Endothelial Venules and Different Regulation of Genes Controlling Lymphocyte Entry to Lymph Nodes
    Authors: K Veerman, C Tardiveau, F Martins, J Coudert, JP Girard
    Cell Rep, 2019;26(11):3116-3131.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. T Cells in Nonlymphoid Tissues Give Rise to Lymph-Node-Resident Memory T Cells
    Authors: LK Beura, S Wijeyesing, EA Thompson, MG Macchietto, PC Rosato, MJ Pierson, JM Schenkel, JS Mitchell, V Vezys, BT Fife, S Shen, D Masopust
    Immunity, 2018;48(2):327-338.e5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018;13(10):e0206756.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
    Authors: M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen
    Nat Commun, 2018;9(1):1511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer
    Authors: M Ouarné, C Bouvard, G Boneva, C Mallet, J Ribeiro, A Desroches-, E Soleilhac, E Tillet, O Peyruchaud, S Bailly
    J. Exp. Clin. Cancer Res., 2018;37(1):209.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1
    Authors: LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson
    Nat. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors
    Authors: T Torcellan, HR Hampton, J Bailey, M Tomura, R Brink, T Chtanova
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration
    Authors: Tsun-Ho Lo
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  10. A reverse signaling pathway downstream of Sema4A controls cell migration via Scrib
    Authors: Tianliang Sun
    J. Cell Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Non-invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle.
    Authors: Thorek D, Ulmert D, Diop N, Lupu M, Doran M, Huang R, Abou D, Larson S, Grimm J
    Nat Commun, 2014;5(0):3097.
  12. Fusing VE-cadherin to alpha-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation.
    Authors: Dartsch N, Schulte D, Hagerling R, Kiefer F, Vestweber D
    Mol Cell Biol, 2014;34(9):1634-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. A micro-sterile inflammation array as an adjuvant for influenza vaccines.
    Authors: Wang J, Shah D, Chen X, Anderson R, Wu M
    Nat Commun, 2014;5(0):4447.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Low molecular weight hyaluronan induces lymphangiogenesis through LYVE-1-mediated signaling pathways.
    Authors: Wu M, Du Y, Liu Y, He Y, Yang C, Wang W, Gao F
    PLoS ONE, 2014;9(3):e92857.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  15. A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.
    Authors: Reichardt, Peter, Patzak, Irene, Jones, Kristian, Etemire, Eloho, Gunzer, Matthias, Hogg, Nancy
    EMBO J, 2013;32(6):829-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus.
    Authors: Zhi L, Kim P, Thompson BD, Pitsillides C, Bankovich AJ, Yun SH, Lin CP, Cyster JG, Wu MX
    J. Immunol., 2011;187(5):2244-51.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Intravital Microscopy
  17. B lymphocytes exit lymph nodes through cortical lymphatic sinusoids by a mechanism independent of sphingosine-1-phosphate-mediated chemotaxis.
    Authors: Sinha RK, Park C, Hwang IY, Davis MD, Kehrl JH
    Immunity, 2009;30(3):434-46.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  18. Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.
    Authors: Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG
    Nat. Immunol., 2009;10(1):58-65.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
    Authors: Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7137-42.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009;119(10):2954-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. CXCR4-gp120-IIIB interactions induce caspase-mediated apoptosis of prostate cancer cells and inhibit tumor growth.
    Authors: Singh S, Bond VC, Powell M, Singh UP, Bumpers HL, Grizzle WE, Lillard JW
    Mol. Cancer Ther., 2009;8(1):178-84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress.
    Authors: Pham TH, Okada T, Matloubian M, Lo CG, Cyster JG
    Immunity, 2007;28(1):122-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor.
    Authors: Backhed F, Crawford PA, O&amp;amp;apos;Donnell D, Gordon JI
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(2):606-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  24. Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells.
    Authors: Phan TG, Grigorova I, Okada T, Cyster JG
    Nat. Immunol., 2007;8(9):992-1000.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells.
    Authors: Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, Sakabe J, Yoshiki R, Tamamura H, Fujii N, Inaba K, Tokura Y
    Am. J. Pathol., 2007;171(4):1249-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Mouse LYVE-1 Antibody
By Anonymous on 12/07/2020
Application: ICC/IF Sample Tested: Lymph node tissue Species: Mouse

LN were fixed in 4% PFA, sectioned and blocked/permeabilized with 0.1% Tx-100, 0.01% NaN3, 1% FBS. Sections were stained overnight (MAB2125) at a dilution of 1:200. Antibody was detected using a secondary FITC-anti rat (1:500).


Mouse LYVE-1 Antibody
By Anonymous on 10/03/2019
Application: IHC Sample Tested: Meninges Species: Mouse

Mouse meninges stained with Lyve1 (1:100, white), CD31 (red), iba1 (green), dapi. Lyve1 labels lymphatic vessels as well as a subset of macrophages.