Mouse LYVE-1 Antibody

(18 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LYVE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD44 or recombinant human LYVE-1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 223322
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF/3 mouse pro-B cell line transfected with mouse LYVE-1
    Ala24-Thr234
    Accession # Q8BHC0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 0.02-0.1 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Hyaluronan to immobilized Recombinant Mouse LYVE-1 (Catalog # 2125-LY) coated at 5 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed LYVE-1 transfected cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse LYVE‑1 by Western Blot. Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for LYVE‑1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Detection of LYVE‑1 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE‑1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a recently identified receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1-3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9-11).

  • References:
    1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
    2. Evered, D. and J. Whelan (1989) Ciba Found. Symp. 143:1.
    3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
    4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
    5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
    6. Carreira, C.M. et al. (2001) Cancer Research 61:8079.
    7. Jackson, D.J. et al. (2001) Trends Immunol. 22:317.
    8. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
    9. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
    10. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
    11. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.
  • Long Name:
    Lymphatic Vessel Endothelial Hyaluronan Receptor 1
  • Entrez Gene IDs:
    10894 (Human); 114332 (Mouse); 293186 (Rat)
  • Alternate Names:
    cell surface retention sequence binding protein-1; Cell surface retention sequence-binding protein 1; CRSBP1; CRSBP-1; extracellular link domain containing 1; extracellular link domain-containing 1; Extracellular link domain-containing protein 1; HAR; Hyaluronic acid receptor; lymphatic vessel endothelial hyaluronan receptor 1; lymphatic vessel endothelial hyaluronic acid receptor 1; LYVE1; LYVE-1; LYVE-1XLKD1; XLKD1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. In vivo photolabeling of tumor-infiltrating cells reveals highly regulated egress of T-cell subsets from tumors
    Authors: T Torcellan, HR Hampton, J Bailey, M Tomura, R Brink, T Chtanova
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  2. Dendritic cells enter lymph vessels by hyaluronan-mediated docking to the endothelial receptor LYVE-1
    Authors: LA Johnson, S Banerji, W Lawrance, U Gileadi, G Prota, KA Holder, YM Roshorm, T Hanke, V Cerundolo, NW Gale, DG Jackson
    Nat. Immunol., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Characterization of the Expression and Function of the C-Type Lectin Receptor CD302 in Mice and Humans Reveals a Role in Dendritic Cell Migration
    Authors: Tsun-Ho Lo
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Non-invasive mapping of deep-tissue lymph nodes in live animals using a multimodal PET/MRI nanoparticle.
    Authors: Thorek D, Ulmert D, Diop N, Lupu M, Doran M, Huang R, Abou D, Larson S, Grimm J
    Nat Commun, 2014;5(0):3097.
  5. Low molecular weight hyaluronan induces lymphangiogenesis through LYVE-1-mediated signaling pathways.
    Authors: Wu M, Du Y, Liu Y, He Y, Yang C, Wang W, Gao F
    PLoS ONE, 2014;9(3):e92857.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. Fusing VE-cadherin to alpha-catenin impairs fetal liver hematopoiesis and lymph but not blood vessel formation.
    Authors: Dartsch N, Schulte D, Hagerling R, Kiefer F, Vestweber D
    Mol Cell Biol, 2014;34(9):1634-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  7. A micro-sterile inflammation array as an adjuvant for influenza vaccines.
    Authors: Wang J, Shah D, Chen X, Anderson R, Wu M
    Nat Commun, 2014;5(0):4447.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  8. A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.
    Authors: Reichardt, Peter, Patzak, Irene, Jones, Kristian, Etemire, Eloho, Gunzer, Matthias, Hogg, Nancy
    EMBO J, 2013;32(6):829-43.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IF
  9. FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus.
    Authors: Zhi L, Kim P, Thompson BD, Pitsillides C, Bankovich AJ, Yun SH, Lin CP, Cyster JG, Wu MX
    J. Immunol., 2011;187(5):2244-51.
    Species: Mouse
    Sample Type: In Vivo
    Application: Intravital imaging
  10. B lymphocytes exit lymph nodes through cortical lymphatic sinusoids by a mechanism independent of sphingosine-1-phosphate-mediated chemotaxis.
    Authors: Sinha RK, Park C, Hwang IY, Davis MD, Kehrl JH
    Immunity, 2009;30(3):434-46.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  11. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009;119(10):2954-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  12. Cortical sinus probing, S1P1-dependent entry and flow-based capture of egressing T cells.
    Authors: Grigorova IL, Schwab SR, Phan TG, Pham TH, Okada T, Cyster JG
    Nat. Immunol., 2009;10(1):58-65.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  13. CXCR4-gp120-IIIB interactions induce caspase-mediated apoptosis of prostate cancer cells and inhibit tumor growth.
    Authors: Singh S, Bond VC, Powell M, Singh UP, Bumpers HL, Grizzle WE, Lillard JW
    Mol. Cancer Ther., 2009;8(1):178-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  14. Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth.
    Authors: Cho WG, Albuquerque RJ, Kleinman ME, Tarallo V, Greco A, Nozaki M, Green MG, Baffi JZ, Ambati BK, De Falco M, Alexander JS, Brunetti A, De Falco S, Ambati J
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(17):7137-42.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. Postnatal lymphatic partitioning from the blood vasculature in the small intestine requires fasting-induced adipose factor.
    Authors: Backhed F, Crawford PA, O&amp;amp;apos;Donnell D, Gordon JI
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(2):606-11.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  16. CXCL12-CXCR4 engagement is required for migration of cutaneous dendritic cells.
    Authors: Kabashima K, Shiraishi N, Sugita K, Mori T, Onoue A, Kobayashi M, Sakabe J, Yoshiki R, Tamamura H, Fujii N, Inaba K, Tokura Y
    Am. J. Pathol., 2007;171(4):1249-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  17. Subcapsular encounter and complement-dependent transport of immune complexes by lymph node B cells.
    Authors: Phan TG, Grigorova I, Okada T, Cyster JG
    Nat. Immunol., 2007;8(9):992-1000.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  18. S1P1 receptor signaling overrides retention mediated by G alpha i-coupled receptors to promote T cell egress.
    Authors: Pham TH, Okada T, Matloubian M, Lo CG, Cyster JG
    Immunity, 2007;28(1):122-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 18 Citations
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