Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 108928
View all Mer Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805

Specificity

Detects mouse Mer in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Mer APC‑conjugated Antibody

Detection of Mer antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Mer APC-conjugated Monoclonal Antibody (Catalog # FAB5912A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Mer APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Reviewed Applications

Read 3 reviews rated 4.7 using FAB5912A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein Growth-Arrest-Specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

References

  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.

Long Name

Receptor Tyrosine Protein Kinase Mer

Alternate Names

c-Eyk, C-mer, MerTK

Entrez Gene IDs

10461 (Human); 17289 (Mouse); 102143798 (Cynomolgus Monkey)

Gene Symbol

MERTK

UniProt

Q60805

Additional Mer Products

Product Documents for Mouse Mer APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Mer APC‑conjugated Antibody

For research use only

Citations for Mouse Mer APC‑conjugated Antibody

Customer Reviews for Mouse Mer APC‑conjugated Antibody (3)

4.7 out of 5
3 Customer Ratings
5 Stars
67%
4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Mouse Mer APC-conjugated Antibody
    Name: Anonymous
    Application: In vivo imaging
    Sample Tested: Liver cells
    Species: Mouse
    Verified Customer | Posted 09/24/2018
    In vivo imaging of liver macrophages using intravital laser confocal microscopy. Ccr2/Cx3cr1 transgenic mice were anaesthetised with ketamine and medetomidine, received i.v. injection of fluorophore-conjugated antibodies [anti-F4/80 (BV421) and anti-MerTK (APC)] and then surgical exposure of the liver was performed. Imaging was performed under isofluorane anaesthesia on a Leica SP5 laser scanning confocal microscope using a 20x objective. Videos were recorded at defined regions of interest. Image analysis was performed using Imaris (v 8.0). Representative screen shots of intravital liver imaging videos showing F4/80+ macrophages (blue) expressing MerTK (pink), ccr2 (red) or cx3cr1 (green), located in centrilobular areas of the liver.
    Mouse Mer APC‑conjugated Antibody FAB5912A
  • Mouse Mer APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: C1498
    Species: Mouse
    Verified Customer | Posted 09/08/2016
  • Mouse Mer APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: PRIMARY MACROPHAGES
    Species: Mouse
    Verified Customer | Posted 07/03/2016

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