Mouse Mer Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Mer in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 108928
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
    Glu23-Phe498
    Accession # Q60805
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mer in J774A.1 Mouse Cell line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Mer Monoclonal Antibody (Catalog # MAB5912, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

  • References:
    1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
    2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
  • Long Name:
    Receptor Tyrosine Protein Kinase Mer
  • Entrez Gene IDs:
    10461 (Human); 17289 (Mouse)
  • Alternate Names:
    c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 58  
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Normal Rat IgG Control

Ctrl 6-001-A 7
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF005 3  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 1  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115
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