Mouse Mer PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB5912P
Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (4)
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Mouse Mer PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Mer in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 108928
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Mer
Glu23-Phe498
Accession # Q60805
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Mer in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse Mer PE‑conjugated Monoclonal Antibody (Catalog # FAB5912P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mer

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a receptor tyrosine kinase subfamily. The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. These receptors bind the vitamin K-dependent protein growth-arrest-specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration or the prevention of apoptosis. Studies suggest that this family of tyrosine kinase receptors may be involved in hematopoiesis, embryonic development, tumorigenesis and regulation of testicular functions (1-2).

References
  1. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
  2. Crosier, K.E. and P.S Crosier (1997) Pathology 29:131.
Long Name
Receptor Tyrosine Protein Kinase Mer
Entrez Gene IDs
10461 (Human); 17289 (Mouse)
Alternate Names
c-Eyk; c-mer proto-oncogene tyrosine kinase; C-mer; EC 2.7.10; EC 2.7.10.1; MER receptor tyrosine kinase; Mer; MerTK; MGC133349; Receptor tyrosine kinase MerTK; RP38Proto-oncogene c-Mer; STK kinase; tyrosine-protein kinase Mer

Product Datasheets

Citations for Mouse Mer PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. TTI-621 (SIRP?Fc), a CD47-blocking cancer immunotherapeutic, triggers phagocytosis of lymphoma cells by multiple polarized macrophage subsets
    Authors: GHY Lin, V Chai, V Lee, K Dodge, T Truong, M Wong, LD Johnson, E Linderoth, X Pang, J Winston, PS Petrova, RA Uger, NN Viller
    PLoS ONE, 2017;12(10):e0187262.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  2. Development of a pHrodo-based assay for the assessment of in vitro and in vivo erythrophagocytosis during experimental trypanosomosis.
    Authors: Stijlemans, Benoit, Cnops, Jennifer, Naniima, Peter, Vaast, Axel, Bockstal, Viki, De Baetselier, Patrick, Magez, Stefan
    PLoS Negl Trop Dis, 2015;9(3):e0003561.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  3. NK-, NKT- and CD8-Derived IFNgamma Drives Myeloid Cell Activation and Erythrophagocytosis, Resulting in Trypanosomosis-Associated Acute Anemia.
    Authors: Cnops J, De Trez C, Stijlemans B, Keirsse J, Kauffmann F, Barkhuizen M, Keeton R, Boon L, Brombacher F, Magez S
    PLoS Pathog, 2015;11(6):e1004964.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  4. GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.
    Authors: Taichman, Russell, Patel, Lalit R, Bedenis, Rachel, Wang, Jingchen, Weidner, Savannah, Schumann, Taibrian, Yumoto, Kenji, Berry, Janice E, Shiozawa, Yusuke, Pienta, Kenneth
    PLoS ONE, 2013;8(4):e61873.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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Mouse Mer PE-conjugated Antibody
By Anonymous on 09/08/2016
Application: Flow Sample Tested: iDC human immature myeloid dendritic cells Species: Human