Mouse Meteorin-like/METRNL PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC6679P-025
Detection of Meteorin‑like/METRNL in Neuro‑2A Mouse Cell Line by Flow Cytometry.
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Mouse Meteorin-like/METRNL PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Meteorin-like/METRNL in direct ELISA.
Source
Monoclonal Rat IgG2B Clone # 829535
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-S-derived recombinant mouse Meteorin-like/METRNL
Gln46-Glu311
Accession # Q8VE43
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Meteorin‑like/METRNL in Neuro‑2A Mouse Cell Line by Flow Cytometry. View Larger

Detection of Meteorin‑like/METRNL in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse Meteorin‑like/METRNL PE‑conjugated Monoclonal Antibody (Catalog # IC6679P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Meteorin-like/METRNL

Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% aa homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).

References
  1. Park, J.A. et al. (2008) Glia 56:247.
  2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
  3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
  4. Nishino, J. et al. (2004) EMBO J. 23:1998.
  5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
  6. Accession # Q8VE43.
  7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
  8. Surace, C. et al. (2009) Clin. Genet. 76:256.
Entrez Gene IDs
284207 (Human); 210029 (Mouse); 316842 (Rat)
Alternate Names
Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788

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