Mouse Meteorin-like/METRNL PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse Meteorin-like/METRNL in direct ELISA.
  • Source
    Monoclonal Rat IgG2B Clone # 829535
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-S-derived recombinant mouse Meteorin-like/METRNL
    Accession # Q8VE43
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Meteorin‑like/METRNL in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse Meteorin‑like/METRNL PE‑conjugated Monoclonal Antibody (Catalog # IC6679P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Meteorin-like/METRNL
Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% aa homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).
  • References:
    1. Park, J.A. et al. (2008) Glia 56:247.
    2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
    3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
    4. Nishino, J. et al. (2004) EMBO J. 23:1998.
    5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
    6. Accession # Q8VE43.
    7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
    8. Surace, C. et al. (2009) Clin. Genet. 76:256.
  • Entrez Gene IDs:
    284207 (Human); 210029 (Mouse); 316842 (Rat)
  • Alternate Names:
    Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; METRNL; MGC99788
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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