Mouse Meteorin-like/METRNL Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse METRNL in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 829535
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse METRNL
    Gln46-Glu311
    Accession # Q8VE43
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Meteorin‑like/METRNL in Neuro‑2A Mouse Cell Line by Flow Cytometry. Neuro‑2A mouse neuroblastoma cell line was stained with Rat Anti-Mouse Meteorin‑like/METRNL Monoclonal Antibody (Catalog # MAB66791, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Meteorin-like/METRNL
Meteorin-like protein (METRNL) is a 311 amino acid (aa) protein that shows 45% homology with Meteorin (in mouse). Meteorin itself is a secreted 30 kDa protein that is expressed in glial and neuronal cells and their precursors during development and in the adult (1‑3). In the adult Meteorin is primarily expressed by astrocytes (3). Meteorin promotes neurite outgrowth, glial cell differentiation, and neuronal survival following excitotoxic injury (2‑5). It also inhibits sprouting angiogenesis and promotes blood vessel maturation (1). Mouse Meteorin‑like protein is synthesized with a 45 amino acid (aa) signal peptide and is selectively expressed in the otic vesicle during inner ear development (6, 7). Mature mouse Meteorin‑like protein shares 79% and 98% aa sequence identity with human and rat Meteorin‑like protein, respectively. Alternate splicing generates an additional isoform that lacks the N‑terminal 82 amino acids including the signal peptide. R&D Systems in‑house testing indicates that Meteorin‑like protein enhances the outgrowth of neurites from cultured mouse cortical neurons. The promoter region of the METRNL gene contains a recognition site for the Pax2, Pax5, and Pax8 transcription factors, and Pax2 binds specifically to this region (7). METRNL is the only gene found within the terminus of the human chromosome 17 q‑arm which can be deleted in the rare Ring 17 syndrome (8).
  • References:
    1. Park, J.A. et al. (2008) Glia 56:247.
    2. Jorgensen, J.R. et al. (2009) J. Mol. Neurosci. 39:104.
    3. Lee, H.S. et al. (2010) J. Cell Sci. 123:1959.
    4. Nishino, J. et al. (2004) EMBO J. 23:1998.
    5. Jorgensen, J.R. et al. (2011) Neurobiol. Dis. 41:160.
    6. Accession # Q8VE43.
    7. Ramialison, M. et al. (2008) Genome Biol. 9:R145.
    8. Surace, C. et al. (2009) Clin. Genet. 76:256.
  • Entrez Gene IDs:
    284207 (Human); 210029 (Mouse); 316842 (Rat)
  • Alternate Names:
    Meteorin like; meteorin, glial cell differentiation regulator-like; meteorin-like protein; Meteorin-like; METRNL; MGC99788
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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