Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 145106
View all MMP-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMP-3
Tyr18-Cys477
Accession # P28862

Specificity

Detects the pro- and mature forms of mouse MMP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, -12, -13, or recombinant mouse MMP-9 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse MMP-3 Antibody (145106)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse MMP‑3 (Catalog # 548-MM)

Reviewed Applications

Read 2 reviews rated 4 using MAB548 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MMP-3

Matrix metalloproteinases are a family of zinc and calcium-dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1) can degrade a broad range of substrates including collagen alpha -chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9 and pro-MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodeling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains, a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like) domain.

Long Name

Matrix Metalloproteinase 3

Alternate Names

MMP3, Stromelysin 1

Entrez Gene IDs

4314 (Human); 17392 (Mouse)

Gene Symbol

MMP3

UniProt

P28862

Additional MMP-3 Products

Product Documents for Mouse MMP-3 Antibody (145106)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse MMP-3 Antibody (145106)

For research use only

Citations for Mouse MMP-3 Antibody (145106)

Customer Reviews for Mouse MMP-3 Antibody (145106) (2)

4 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
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  • Mouse MMP-3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 11/10/2021
    Mouse MMP-3 Antibody (145106) MAB548
  • Mouse MMP-3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tissue Lysates
    Species: Mouse
    Verified Customer | Posted 02/08/2017
    Antibody detects the active form of MMP-3 (45 kD)
    Mouse MMP-3 Antibody (145106) MAB548

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