Mouse MMP-3 Antibody

Catalog # Availability Size / Price Qty
MAB548
MAB548-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Mouse MMP-3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects the pro- and mature forms of mouse MMP-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -10, -12, -13, or recombinant mouse MMP-9 is observed.
Source
Monoclonal Rat IgG2A Clone # 145106
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMP-3
Tyr18-Cys477
Accession # P28862
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse MMP‑3 (Catalog # 548-MM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-3

Matrix metalloproteinases are a family of zinc and calcium-dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-3 (stromelysin-1) can degrade a broad range of substrates including collagen alpha -chains, aggrecan, laminin, fibronectin, elastin, casein, alpha -1 antitrypsin, myelin basic protein, IL-1 beta, IGFBP-3, pro-MMP-1, pro-MMP-7, pro-MMP-8, pro-MMP-9 and pro-MMP-13. MMP-3 does not cleave the triple helical region of interstitial collagens, a characteristic which distinguishes the stromelysins from the collagenases. The MMP-3 substrate repertoire extends beyond extracellular matrix proteins and implicates MMP-3 in roles other than direct tissue remodeling, for instance, enzyme cascades and cytokine regulation. MMP-3 is expressed by fibroblasts, chrondrocytes, osteoblasts, endothelial cells, smooth muscle cells and macrophages. Structurally, MMP-3 may be divided into several distinct domains, a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site, a short hinge region, and a carboxyl terminal (hemopexin-like) domain.

Long Name
Matrix Metalloproteinase 3
Entrez Gene IDs
4314 (Human); 17392 (Mouse)
Alternate Names
CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1

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Citations for Mouse MMP-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia.
    Authors: Crocker SJ, Frausto RF, Whitton JL, Milner R
    Glia, 2008-08-15;56(0):1187-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Interleukin-1 receptor type I signaling critically regulates infarct healing and cardiac remodeling.
    Authors: Bujak M, Dobaczewski M, Chatila K, Mendoza LH, Li N, Reddy A, Frangogiannis NG
    Am. J. Pathol., 2008-06-05;173(1):57-67.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Blood-brain barrier disruption by stromelysin-1 facilitates neutrophil infiltration in neuroinflammation.
    Authors: Gurney KJ, Estrada EY, Rosenberg GA
    Neurobiol. Dis., 2006-04-19;23(1):87-96.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Induction of host matrix metalloproteinases by Borrelia burgdorferi differs in human and murine lyme arthritis.
    Authors: Behera AK, Hildebrand E, Scagliotti J, Steere AC, Hu LT
    Infect. Immun., 2005-01-01;73(1):126-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Reviews for Mouse MMP-3 Antibody

Average Rating: 4 (Based on 2 Reviews)

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Mouse MMP-3 Antibody
By Anonymous on 11/10/2021
Application: WB Sample Tested: Cell Lysates Species: Mouse

Mouse MMP-3 Antibody
By Anonymous on 02/08/2017
Application: WB Sample Tested: Tissue Lysates Species: Mouse

Antibody detects the active form of MMP-3 (45 kD)