Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
View all MMR/CD206 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q2HZ94

Specificity

Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human MMR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse MMR/CD206 APC‑conjugated Antibody

Detection of MMR/CD206 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse MMR/CD206 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2535A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse MMR/CD206 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Reviewed Applications

Read 3 reviews rated 4 using FAB2535A in the following applications:

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
  4. Harris, N. et al. (1992) Blood 80:2363.
  5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Gene Symbol

MRC1

UniProt

Q2HZ94

Additional MMR/CD206 Products

Product Documents for Mouse MMR/CD206 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse MMR/CD206 APC‑conjugated Antibody

For research use only

Citations for Mouse MMR/CD206 APC‑conjugated Antibody

Customer Reviews for Mouse MMR/CD206 APC‑conjugated Antibody (3)

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Mouse MMR/CD206 APC-conjugated Antibody
    Name: James Xiao
    Application: Immunohistochemistry-Frozen
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 05/01/2018
    Red: Mouse MMR/CD206 APC Blue: Nuclei
    Antibody dilution: 1:100 in a serum-free blocking buffer (DAKO); Some non-specific bindings were detected in RBCs
    Mouse MMR/CD206 APC‑conjugated Antibody FAB2535A
  • Mouse MMR/CD206 APC-conjugated Antibody
    Name: Jim Hsiao
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 04/27/2018
    Mouse MMR/CD206 APC‑conjugated Antibody FAB2535A
  • Mouse MMR/CD206 APC-conjugated Antibody
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: lung
    Species: Mouse
    Verified Customer | Posted 04/27/2018
    Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets.
    A single cell suspension of a mouse lung was blocked with Fc block for 30 minutes. Cells were then stained with the Mouse MMR/CD206 APC-conjugated Antibody (1:100 ug) in FACS buffer for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.
    Mouse MMR/CD206 APC‑conjugated Antibody FAB2535A

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