Mouse MMR/CD206 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2535P
Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (9)
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Mouse MMR/CD206 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human MMR is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q2HZ94
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MMR/CD206 antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of MMR/CD206 in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse MMR/CD206 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2535P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMR/CD206

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References
  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
  4. Harris, N. et al. (1992) Blood 80:2363.
  5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Long Name
Macrophage Mannose Receptor
Entrez Gene IDs
4360 (Human); 17533 (Mouse); 291327 (Rat)
Alternate Names
CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1

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Citations for Mouse MMR/CD206 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Inflammasome Coordinates Senescent Chronic Wound Induced by Thalassophryne nattereri Venom
    Authors: Lima, C;Andrade-Barros, AI;Carvalho, FF;Falc�o, MAP;Lopes-Ferreira, M;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Chronic exposure to biomass ambient particulate matter triggers alveolar macrophage polarization and activation in the rat lung
    Authors: S Wang, Y Chen, W Hong, B Li, Y Zhou, P Ran
    Journal of Cellular and Molecular Medicine, 2022-01-06;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Anti-HER2 induced myeloid cell alterations correspond with increasing vascular maturation in a murine model of HER2+ breast cancer
    Authors: MJ Bloom, AM Jarrett, TA Triplett, AK Syed, T Davis, TE Yankeelov, AG Sorace
    BMC Cancer, 2020-04-28;20(1):359.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019-02-06;22(0):101116.
    Species: Mouse
    Sample Types: Xenograft
    Applications: Flow Cytometry
  5. Deletion of lactate dehydrogenase-A in myeloid cells triggers antitumor immunity
    Authors: P Seth, E Csizmadia, A Hedblom, M Vuerich, H Xie, M Li, MS Longhi, B Wegiel
    Cancer Res., 2017-04-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Differential Immunometabolic Phenotype in Th1 and Th2 Dominant Mouse Strains in Response to High-Fat Feeding.
    Authors: Jovicic N, Jeftic I, Jovanovic I, Radosavljevic G, Arsenijevic N, Lukic M, Pejnovic N
    PLoS ONE, 2015-07-28;10(7):e0134089.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Obesity alters adipose tissue macrophage iron content and tissue iron distribution.
    Authors: Orr J, Kennedy A, Anderson-Baucum E, Webb C, Fordahl S, Erikson K, Zhang Y, Etzerodt A, Moestrup S, Hasty A
    Diabetes, 2013-10-15;63(2):421-32.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Alveolar epithelial cells are critical in protection of the respiratory tract by secretion of factors able to modulate the activity of pulmonary macrophages and directly control bacterial growth.
    Authors: Chuquimia O, Petursdottir D, Periolo N, Fernandez C
    Infect Immun, 2012-11-12;81(1):381-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype.
    Authors: Ylostalo J, Bartosh T, Coble K, Prockop D
    Stem Cells, 2012-10-01;30(10):2283-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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