Mouse MMR/CD206 Antibody

(13 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 45% cross-reactivity with recombinant human MMR is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
    Leu19-Ala1388
    Accession # Q2HZ94
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    0.25 µg/106 cells
    J774 mouse monocyte/macrophage cell line
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Immunohistochemistry
MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMR/CD206

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

  • References:
    1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
    2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
    3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
    4. Harris, N. et al. (1992) Blood 80:2363.
    5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
    6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
    7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
    8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
  • Long Name:
    Macrophage Mannose Receptor
  • Entrez Gene IDs:
    4360 (Human); 17533 (Mouse); 291327 (Rat)
  • Alternate Names:
    CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
    Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment
    Authors: JC Gensel, TJ Kopper, B Zhang, MB Orr, WM Bailey
    Sci Rep, 2017;7(0):40144.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  3. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
    Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
    Authors: T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou
    Sci Rep, 2017;7(1):8641.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  5. Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
    Sci Rep, 2016;6(0):36698.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  6. miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair
    J Neurosci, 2016;36(32):8516-32.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
    Front Cell Neurosci, 2016;10(0):286.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
    Authors: Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, Jansen M, Vitek M, Gunn M, Colton C
    J Neurosci, 2015;35(15):5969-82.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  9. Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.
    Authors: Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou A, Leak R, Yu X, Chen L, Chen J
    Sci Rep, 2015;5(0):9621.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  10. Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.
    Authors: Ko S, Ladanyi A, Lengyel E, Naora H
    Am J Pathol, 2014;184(1):271-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.
    Authors: Pepe, Giovanna, Calderazzi, Giorgia, De Maglie, Marcella, Villa, Alessand, Vegeto, Elisabet
    J Neuroinflammation, 2014;11(1):211.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. MicroRNA-124 as a novel treatment for persistent hyperalgesia.
    J Neuroinflammation, 2012;9(0):143.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  13. p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
    Authors: Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
    Am. J. Pathol., 2009;174(1):153-63.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 13 Citations
Cell and Tissue Staining Kits
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Isotype Controls
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Normal Goat IgG Control

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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Chicken Anti-Goat IgG Biotinylated Antibody

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Average Rating: 5 (Based on 1 review)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse MMR/CD206 Antibody AF2535
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 WB Mouse Anonymous 09/26/2016
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Western Blot Mouse MMR/CD206 Antibody AF2535
Western Blot: Mouse MMR/CD206 Antibody [AF2535]

Summary

ApplicationWestern Blot
Sample TestedLiver tissue
SpeciesMouse

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