Mouse MMR/CD206 Antibody
Mouse MMR/CD206 Antibody Summary
Leu19-Ala1388
Accession # Q2HZ94
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMR/CD206
The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).
- East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364.
- Chieppa, M. et al. (2003) J. Immunol. 171:4552.
- Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
- Harris, N. et al. (1992) Blood 80:2363.
- Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
- Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
- Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
- Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Product Datasheets
Citations for Mouse MMR/CD206 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury
Authors: JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond
J Neuroinflammation, 2018;15(1):192.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex
Authors: K Hashimoto, M Nakashima, A Hamano, M Gotoh, H Ikeshima-K, K Murakami-M, Y Miyamoto
Sci Rep, 2018;8(1):9715.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia
Authors: X Liu, S Wen, F Yan, K Liu, L Liu, L Wang, S Zhao, X Ji
J Neuroinflammation, 2018;15(1):39.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
Authors: T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou
Sci Rep, 2017;7(1):8641.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC OCT-embedded -
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using (111)In-tilmanocept
Authors: Z Varasteh, F Hyafil, N Anizan, D Diallo, R Aid-Launai, S Mohanta, Y Li, M Braeuer, K Steiger, J Vigne, Z Qin, SG Nekolla, JE Fabre, Y Döring, D Le Guludec, A Habenicht, DR Vera, M Schwaiger
EJNMMI Res, 2017;7(1):40.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment
Authors: JC Gensel, TJ Kopper, B Zhang, MB Orr, WM Bailey
Sci Rep, 2017;7(0):40144.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen -
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
J. Immunol, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow -
miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair
J Neurosci, 2016;36(32):8516-32.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen -
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen -
Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
Front Cell Neurosci, 2016;10(0):286.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen -
Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.
Authors: Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou A, Leak R, Yu X, Chen L, Chen J
Sci Rep, 2015;5(0):9621.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Not Specified -
Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
Authors: Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, Jansen M, Vitek M, Gunn M, Colton C
J Neurosci, 2015;35(15):5969-82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen -
Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.
Authors: Ko S, Ladanyi A, Lengyel E, Naora H
Am J Pathol, 2014;184(1):271-81.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow -
Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.
Authors: Pepe, Giovanna, Calderazzi, Giorgia, De Maglie, Marcella, Villa, Alessand, Vegeto, Elisabet
J Neuroinflammation, 2014;11(1):211.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB -
MicroRNA-124 as a novel treatment for persistent hyperalgesia.
J Neuroinflammation, 2012;9(0):143.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
Authors: Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
Am. J. Pathol., 2009;174(1):153-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded
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