Mouse MMR/CD206 Antibody
Mouse MMR/CD206 Antibody Summary
Leu19-Ala1388
Accession # Q2HZ94
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
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Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMR/CD206
The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).
- East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364.
- Chieppa, M. et al. (2003) J. Immunol. 171:4552.
- Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
- Harris, N. et al. (1992) Blood 80:2363.
- Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
- Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
- Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
- Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.
Product Datasheets
Citations for Mouse MMR/CD206 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke
Authors: M Hu, Y Lin, X Men, S Wang, X Sun, Q Zhu, D Lu, S Liu, B Zhang, W Cai, Z Lu
Journal of Neuroinflammation, 2021;18(1):90.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Differentiated glioblastoma cells accelerate tumor progression by shaping the tumor microenvironment via CCN1-mediated macrophage infiltration
Authors: A Uneda, K Kurozumi, A Fujimura, K Fujii, J Ishida, Y Shimazu, Y Otani, Y Tomita, Y Hattori, Y Matsumoto, N Tsuboi, K Makino, S Hirano, A Kamiya, I Date
Acta neuropathologica communications, 2021;9(1):29.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia
Authors: Z Germana, G Simona, L Marialucia, M Biagina, V Christine, F Paola, C Matteo, C Pasquale, M Davide, T Mario, M Massimilia, G Carlo, S Gaia, M Fabio
Cell Death & Disease, 2021;12(5):435.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Astragaloside IV promotes microglia/macrophages M2 polarization and enhances neurogenesis and angiogenesis through PPAR&gamma pathway after cerebral ischemia/reperfusion injury in rats
Authors: L Li, H Gan, H Jin, Y Fang, Y Yang, J Zhang, X Hu, L Chu
International immunopharmacology, 2021;92(0):107335.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Imaging of Inflammation in Spinal Cord Injury: Novel Insights on the Usage of PFC-Based Contrast Agents
Authors: F Garello, M Boido, M Miglietti, V Bitonto, M Zenzola, M Filippi, F Arena, L Consolino, M Ghibaudi, E Terreno
Biomedicines, 2021;9(4):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction
Authors: L Zhang, J Chen, L Yan, Q He, H Xie, M Chen
Frontiers in Pharmacology, 2021;12(0):646240.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface
Authors: A Chu, SY Kok, J Tsui, MC Lin, B Aguirre, M Wadehra
Journal of reproductive immunology, 2021;145(0):103309.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Bone marrow mesenchymal stem cell-derived exosomal microRNA-125a promotes M2 macrophage polarization in spinal cord injury by downregulating IRF5
Authors: Q Chang, Y Hao, Y Wang, Y Zhou, H Zhuo, G Zhao
Brain research bulletin, 2021;170(0):199-210.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages
Authors: A Menga, M Serra, S Todisco, C Riera-Domi, U Ammarah, M Ehling, EM Palmieri, MA Di Noia, R Gissi, M Favia, CL Pierri, PE Porporato, A Castegna, M Mazzone
EMBO Mol Med, 2020;0(0):e11210.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Transient microglial absence assists postmigratory cortical neurons in proper differentiation
Authors: Y Hattori, Y Naito, Y Tsugawa, S Nonaka, H Wake, T Nagasawa, A Kawaguchi, T Miyata
Nat Commun, 2020;11(1):1631.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The glycosyltransferase EXTL2 promotes proteoglycan deposition and injurious neuroinflammation following demyelination
Authors: A Pu, MK Mishra, Y Dong, S Ghorbaniga, EL Stephenson, KS Rawji, C Silva, H Kitagawa, S Sawcer, VW Yong
J Neuroinflammation, 2020;17(1):220.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration
Authors: F Jiang, W Zhang, M Zhou, Z Zhou, M Shen, N Chen, X Jiang
Theranostics, 2020;10(14):6216-6230.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophages
Authors: D Wang, F Liu, L Zhu, P Lin, F Han, X Wang, X Tan, L Lin, Y Xiong
J Neuroinflammation, 2020;17(1):257.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer's disease mice
Authors: Y Wang, Y Lin, L Wang, H Zhan, X Luo, Y Zeng, W Wu, X Zhang, F Wang
Aging (Albany NY), 2020;12(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Therapeutic Effects of Human Mesenchymal Stem Cells in a Mouse Model of Cerebellar Ataxia with Neuroinflammation
Authors: Y Nam, D Yoon, J Hong, MS Kim, TY Lee, KS Kim, HW Lee, K Suk, SR Kim
J Clin Med, 2020;9(11):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Deficiency of microglial Hv1 channel is associated with activation of autophagic pathway and ROS production in LPC-induced demyelination mouse model
Authors: M Chen, LL Yang, ZW Hu, C Qin, LQ Zhou, YL Duan, DB Bosco, LJ Wu, KB Zhan, SB Xu, DS Tian
J Neuroinflammation, 2020;17(1):333.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Skewed macrophage polarization in aging skeletal muscle
Authors: CY Cui, RK Driscoll, Y Piao, CW Chia, M Gorospe, L Ferrucci
Aging Cell, 2019;0(0):e13032.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Microglial VPS35 deficiency regulates microglial polarization and decreases ischemic stroke-induced damage in the cortex
Authors: SY Ye, JE Apple, X Ren, FL Tang, LL Yao, YG Wang, L Mei, YG Zhou, WC Xiong
J Neuroinflammation, 2019;16(1):235.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Wnt canonical pathway activator TWS119 drives microglial anti-inflammatory activation and facilitates neurological recovery following experimental stroke
Authors: D Song, X Zhang, J Chen, X Liu, J Xue, L Zhang, X Lan
J Neuroinflammation, 2019;16(1):256.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance
Authors: S Morita-Tak, K Nakahara, S Hasegawa-I, A Isonishi, K Tatsumi, H Okuda, T Tanaka, M Kitabatake, T Ito, A Wanaka
J Neuroinflammation, 2019;16(1):39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury
Authors: V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix
Nat Commun, 2019;10(1):518.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model
Authors: T Koizumi, K Taguchi, I Mizuta, H Toba, M Ohigashi, O Onishi, K Ikoma, S Miyata, T Nakata, M Tanaka, S Foulquier, HWM Steinbusch, T Mizuno
J Neuroinflammation, 2019;16(1):79.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Iron nanoparticle-labeled murine mesenchymal stromal cells in an osteoarthritic model persists and suggests anti-inflammatory mechanism of action
Authors: AM Hamilton, WY Cheung, A Gómez-Aris, A Sharma, S Nakamura, A Chaboureau, S Bhatt, R Rabani, M Kapoor, PJ Foster, S Viswanatha
PLoS ONE, 2019;14(12):e0214107.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice
Authors: W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen
JCI Insight, 2019;4(20):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model
Authors: G Liu, P Lu, L Chen, W Zhang, M Wang, D Li, X Zhang
Eye (Lond), 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Serelaxin Induces Notch1 Signaling and Alleviates Hepatocellular Damage in Orthotopic Liver Transplantation
Authors: S Kageyama, K Nakamura, B Ke, RW Busuttil, JW Kupiec-Weg
Am. J. Transplant., 2018;0(0):.
Species: Mouse
Sample Types: Protein
Applications: Western Blot -
Intranasal stem cell treatment as a novel therapy for subarachnoid hemorrhage
Authors: CH Nijboer, E Kooijman, CT van Veltho, E Van Tilbor, IA Tiebosch, N Eijkelkamp, RM Dijkhuizen, J Kesecioglu, C Heijnen
Stem Cells Dev., 2018;0(0):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway
Authors: C Qin, Q Liu, ZW Hu, LQ Zhou, K Shang, DB Bosco, LJ Wu, DS Tian, W Wang
Theranostics, 2018;8(19):5434-5451.
Species: Mouse
Sample Types: Cell Lysates
Applications: ICC -
Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice
Authors: J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji
EBioMedicine, 2018;35(0):29-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury
Authors: JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond
J Neuroinflammation, 2018;15(1):192.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex
Authors: K Hashimoto, M Nakashima, A Hamano, M Gotoh, H Ikeshima-K, K Murakami-M, Y Miyamoto
Sci Rep, 2018;8(1):9715.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia
Authors: X Liu, S Wen, F Yan, K Liu, L Liu, L Wang, S Zhao, X Ji
J Neuroinflammation, 2018;15(1):39.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
Authors: T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou
Sci Rep, 2017;7(1):8641.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
J. Immunol, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment
Authors: JC Gensel, TJ Kopper, B Zhang, MB Orr, WM Bailey
Sci Rep, 2017;7(0):40144.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using (111)In-tilmanocept
Authors: Z Varasteh, F Hyafil, N Anizan, D Diallo, R Aid-Launai, S Mohanta, Y Li, M Braeuer, K Steiger, J Vigne, Z Qin, SG Nekolla, JE Fabre, Y Döring, D Le Guludec, A Habenicht, DR Vera, M Schwaiger
EJNMMI Res, 2017;7(1):40.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
Front Cell Neurosci, 2016;10(0):286.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair
J Neurosci, 2016;36(32):8516-32.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
Authors: Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, Jansen M, Vitek M, Gunn M, Colton C
J Neurosci, 2015;35(15):5969-82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.
Authors: Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou A, Leak R, Yu X, Chen L, Chen J
Sci Rep, 2015;5(0):9621.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.
Authors: Pepe, Giovanna, Calderazzi, Giorgia, De Maglie, Marcella, Villa, Alessand, Vegeto, Elisabet
J Neuroinflammation, 2014;11(1):211.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.
Authors: Ko S, Ladanyi A, Lengyel E, Naora H
Am J Pathol, 2014;184(1):271-81.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
MicroRNA-124 as a novel treatment for persistent hyperalgesia.
J Neuroinflammation, 2012;9(0):143.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
Authors: Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
Am. J. Pathol., 2009;174(1):153-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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