Mouse MMR/CD206 Antibody

Name: Mouse MMR/CD206 Antibody
Brand: R&D Systems
Rating: 5 (3 reviews)

AF2535 19 Citations 3 Reviews Datasheet / COA / SDS

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AF2535
AF2535-SP

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Detection of Mouse MMR/CD206 by Western Blot.

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Product Details

Citations (19)

FAQs

Supplemental Products

Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse MMR/CD206 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q2HZ94

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

J774 mouse monocyte/macrophage cell line

Immunohistochemistry

5-15 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Mouse MMR/CD206 by Western Blot. Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

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MMR/CD206 in Mouse Testis. MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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MMR/CD206 in Mouse Lung. MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMR/CD206

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca²⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

References

East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364.
Chieppa, M. et al. (2003) J. Immunol. 171:4552.
Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
Harris, N. et al. (1992) Blood 80:2363.
Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Long Name

Macrophage Mannose Receptor

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Alternate Names

CD206; CLEC13D; CLEC13Dmacrophage mannose receptor 1; C-type lectin domain family 13 member D; mannose receptor, C type 1; MMR; MMRCD206 antigen; MRC1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse MMR/CD206 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway
Authors: C Qin, Q Liu, ZW Hu, LQ Zhou, K Shang, DB Bosco, LJ Wu, DS Tian, W Wang
Theranostics, 2018;8(19):5434-5451.
Species: Mouse
Sample Types: Cell Lysates
Applications: ICC
2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex
Authors: K Hashimoto, M Nakashima, A Hamano, M Gotoh, H Ikeshima-K, K Murakami-M, Y Miyamoto
Sci Rep, 2018;8(1):9715.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice
Authors: J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji
EBioMedicine, 2018;35(0):29-39.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury
Authors: JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond
J Neuroinflammation, 2018;15(1):192.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia
Authors: X Liu, S Wen, F Yan, K Liu, L Liu, L Wang, S Zhao, X Ji
J Neuroinflammation, 2018;15(1):39.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment
Authors: JC Gensel, TJ Kopper, B Zhang, MB Orr, WM Bailey
Sci Rep, 2017;7(0):40144.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen
Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using (111)In-tilmanocept
Authors: Z Varasteh, F Hyafil, N Anizan, D Diallo, R Aid-Launai, S Mohanta, Y Li, M Braeuer, K Steiger, J Vigne, Z Qin, SG Nekolla, JE Fabre, Y Döring, D Le Guludec, A Habenicht, DR Vera, M Schwaiger
EJNMMI Res, 2017;7(1):40.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice
Authors: SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler
J. Immunol, 2017;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow
ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
J. Neurosci., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
HDAC3 inhibition ameliorates spinal cord injury by immunomodulation
Authors: T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou
Sci Rep, 2017;7(1):8641.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC OCT-embedded

miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair
J Neurosci, 2016;36(32):8516-32.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen

Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3?
Sci Rep, 2016;6(0):36698.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen

Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury
Front Cell Neurosci, 2016;10(0):286.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen

Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
Authors: Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, Jansen M, Vitek M, Gunn M, Colton C
J Neurosci, 2015;35(15):5969-82.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Frozen

Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury.
Authors: Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou A, Leak R, Yu X, Chen L, Chen J
Sci Rep, 2015;5(0):9621.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Not Specified

Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype.
Authors: Ko S, Ladanyi A, Lengyel E, Naora H
Am J Pathol, 2014;184(1):271-81.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow

Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4.
Authors: Pepe, Giovanna, Calderazzi, Giorgia, De Maglie, Marcella, Villa, Alessand, Vegeto, Elisabet
J Neuroinflammation, 2014;11(1):211.
Species: Mouse
Sample Types: Cell Lysates
Applications: WB

MicroRNA-124 as a novel treatment for persistent hyperalgesia.
J Neuroinflammation, 2012;9(0):143.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia.
Authors: Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH
Am. J. Pathol., 2009;174(1):153-63.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded