< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.
The Quantikine Mouse MSP/MST1 Immunoassay is a 4.5 hour solid phase ELISA
designed to measure mouse MSP in cell culture supernates, tissue lysates,
serum, plasma, and urine. It contains CHO cell-expressed recombinant mouse MSP
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant mouse MSP.
Results obtained using natural mouse MSP showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural mouse MSP.
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components.
The recovery of mouse MSP spiked to levels throughout the range
of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Samples (n=4)
Tissue Homogenates (n=4)
To assess the linearity of the assay, samples containing and/or spiked
with high concentrations of mouse MSP were serially diluted with the
appropriate Calibrator Diluent to produce samples with values within
the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Macrophage-stimulating protein (MSP), also known as Hepatocyte Growth Factor-like Protein (HGFL) and Scatter Factor-2 (SF2), is a member of the HGF family of growth factors. MSP is secreted as a single chain inactive precursor (pro-MSP). In response to injury, pro-MSP is activated by proteolytic cleavage. Activation results in the creation of alpha and beta chains joined by a disulfide bond.
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