Macrophage-stimulating protein (MSP), also known as Hepatocyte Growth Factor-like Protein (HGFL) and Scatter Factor-2 (SF2), is a member of the HGF family of growth factors. MSP is secreted as a single chain inactive precursor (pro-MSP). In response to injury, pro-MSP is activated by proteolytic cleavage. Activation results in the creation of alpha and beta chains joined by a disulfide bond.
Loading...
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
0.019 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Loading...
Product Summary for Mouse MSP/MST1 Quantikine ELISA Kit
The Quantikine Mouse MSP/MST1 Immunoassay is a 4.5 hour solid phase ELISA
designed to measure mouse MSP in cell culture supernates, tissue lysates,
serum, plasma, and urine. It contains CHO cell-expressed recombinant mouse MSP
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant mouse MSP.
Results obtained using natural mouse MSP showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural mouse MSP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse MSP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.504 | 1.28 | 3.98 | 0.470 | 1.24 | 3.74 |
| Standard Deviation | 0.027 | 0.068 | 0.154 | 0.015 | 0.044 | 0.228 |
| CV% | 5.4 | 5.3 | 3.9 | 3.2 | 3.6 | 6.1 |
Recovery for Mouse MSP/MST1 Quantikine ELISA Kit
The recovery of mouse MSP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 98 | 90-108 |
| Tissue Homogenates (n=4) | 101 | 90-109 |
| Urine (n=4) | 96 | 88-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MSP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MSP/MST1
Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Alternate Names
HGFL, MST1, SF2
Gene Symbol
MST1
Additional MSP/MST1 Products
Product Documents for Mouse MSP/MST1 Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse MSP/MST1 Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Customer Reviews for Mouse MSP/MST1 Quantikine ELISA Kit
There are currently no reviews for this product. Be the first to review Mouse MSP/MST1 Quantikine ELISA Kit and earn rewards!
Have you used Mouse MSP/MST1 Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
View specific protocols for Mouse MSP/MST1 Quantikine ELISA Kit (MMSP0):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
100 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars