Mouse Olfactomedin‑1/Noelin‑1 Alexa Fluor™ Plus 647‑conjugated Antibody

Olfactomedin‑1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as Olfactomedin-1 or pancortin, is a 75 kDa secreted extracellular matrix glycoprotein expressed in the brain (1‑3). Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1‑4) that are combinations of a common central region with alternate N- and C-termini (3, 4). Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled-coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain (4). In the species tested, these isoforms appear to oppose actions of the longer isoforms (5). All isoforms are capable of forming homo‑ or heterodimers through cysteines in their common central region (2). The C‑terminus of Noelin-1 (mouse aa 482‑485) contains a putative endoplasmic reticulum retention sequence that has not been included in the R&D Systems protein (6). Mouse Noelin-1 (aa 17‑481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species (1). In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells (7). Overproduction of Noelin-1 prolongs neural crest cell formation (7). Noelin-1 is also expressed in mouse neural crest, but not frog (1‑3). All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia (1‑3).