Mouse Patched 2/PTCH2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Patched 2/PTCH2 in ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Patched 1 or recombinant human Patched 2 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 865619
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Patched 2/PTCH2
    Glu79-Ser394
    Accession # O35595
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Patched 2/PTCH2 in Mouse Testis. Patched 2/PTCH2 was detected in immersion fixed frozen sections of mouse testis using Rat Anti-Mouse Patched 2/PTCH2 Monoclonal Antibody (Catalog # MAB8096) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Patched 2/PTCH2
Patched 2 (Ptch2) is a 180-200 kDa 12 transmembrane member of the Patched family. It is less widely expressed than Patched 1, but is found at high levels in skin and spermatocytes. The 1182 amino acid (aa) mouse Patched 2 contains two terminal cytoplasmic domains between aa 1-57 and aa 1108-1182. Together with Smoothened (SMO), Patched 2 forms a membrane complex that serves as a receptor for Sonic Hedgehog (Shh). Over the first extracellular loop (aa 79-394), mouse Patched 2 shares 95% aa sequence identity with human Patched 2. Mouse Patched 2 has one potential 1117 aa isoform that lacks aa 207-271.
  • Entrez Gene IDs:
    8643 (Human); 19207 (Mouse)
  • Alternate Names:
    patched (Drosophila) homolog 2; Patched 2; patched homolog 2 (Drosophila); patched homolog 2; protein patched homolog 2; ptc2; PTCH2
Related Research Areas
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Rat HRP-DAB Cell & Tissue Staining Kit

CTS017 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control

Ctrl 6-001-A 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG2A Isotype Control

Ctrl MAB006R  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG Antibody

WB AF005 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse Patched 2/PTCH2 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information