Mouse PD-1 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7738G
Detection of PD‑1 in Mouse Thymocytes by Flow Cytometry.
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Mouse PD-1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PD-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PD-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 766104
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PD-1
Leu25-Gln167
Accession # Q02242
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
Mouse splenocytes treated with PHA and mouse thymocytes gated on CD3+ CD8+ cells or EL-4 cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PD‑1 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of PD‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes gated on CD3+CD8+cells were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405-conjugated Monoclonal Antibody (FAB554V) and either (A) Rat Anti-Mouse PD-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7738G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (IC006G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of PD-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of PD‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) resting or (B) treated with 5 µg/mL of PHA for 72 hours were stained with Rat Anti-Mouse PD-1 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7738G, filled histogram) or isotype control antibody (IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of PD‑1 in EL‑4 cells by Flow Cytometry EL‑4 cells were stained with Rat Anti-Mouse PD‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7738G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4).

References
  1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
  2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  5. Latchman Y. et al. (2001) Nat. Immunol. 2:261.
  6. Tamura, H. et al. (2001) Blood 97:1809.
Long Name
Programmed Death-1
Entrez Gene IDs
5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names
CD279 antigen; CD279; hPD-1; PD1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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