Mouse PD-1 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PD-1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human PD-1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 766104
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PD-1
    Leu25-Gln167
    Accession # Q02242
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of PD‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) resting or (B) treated with 5 μg/mLof PHA for 72 hours were stained with Rat Anti-Mouse PD‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7738G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins
Detection of PD‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes gated on CD3+ CD8+ cells were stained with Rat Anti-Mouse CD4 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB554V) and either (A) Rat Anti-Mouse PD‑1 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB7738G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PD-1

Programmed Death-1 (PD-1) is a type I transmembrane protein belonging to the CD28/CTLA-4 family of immunoreceptors that mediate signals for regulating immune responses (1). Other members of this family include CD28, CTLA-4, and ICOS (2-4). PD-1 is most closely related to CTLA-4 and shares approximately 24% amino acid (aa) sequence identity. The mouse PD-1 gene encodes a 288 aa protein with a putative 20 aa signal peptide, a 149 aa extracellular region with one immunoglobulin-like V-type domain, a 21 aa transmembrane domain, and a 98 aa cytoplasmic region. The cytoplasmic tail contains two tyrosine residues that form the immunoreceptor tyrosine-based inhibitory motif (ITIM) and immunoreceptor tyrosine-based switch motif (ITSM) that are important in mediating PD-1 signaling. Mouse and human PD-1 share approximately 69% aa sequence identity. Two B7 family proteins, PD-L1 (also called B7-H1) and PD-L2, have been identified as PD-1 ligands (5, 6). PD-1 is expressed on activated T cells, B cells, myeloid cells, and on a subset of thymocytes. PD-1 deficient mice have a defect in peripheral tolerance and spontaneously develop autoimmune diseases. Binding of PD-1 to PD-L1 or PD-L2 results in the inhibition of TCR-mediated proliferation and cytokine production as well as BCR-mediated signaling. PD-1 likely has an inhibitory role in regulating immune responses (1-4).

  • References:
    1. Ishida, Y. et al. (1992) EMBO J. 11:3887.
    2. Sharpe, A.H. and G.J. Freeman (2002) Nat. Rev. Immunol. 2:116.
    3. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
    4. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
    5. Latchman Y. et al. (2001) Nat. Immunol. 2:261.
    6. Tamura, H. et al. (2001) Blood 97:1809.
  • Long Name:
    Programmed Death-1
  • Entrez Gene IDs:
    5133 (Human); 18566 (Mouse); 301626 (Rat); 486213 (Canine); 102123659 (Cynomolgus Monkey)
  • Alternate Names:
    CD279 antigen; CD279; hPD-1; PD-1; PD1hPD-l; PDCD1; programmed cell death 1; programmed cell death protein 1; Protein PD-1; SLEB2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 488‑conjugated Isotype Control

Ctrl IC006G  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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