Mouse PIR-B APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2754A
Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry.
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Mouse PIR-B APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.
Source
Monoclonal Rat IgG2A Clone # 326414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
Gly24-Gly635
Accession # AAH26937
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PIR-B antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Anti-Mouse CD19 PE-conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse PIR-B APC-conjugated Monoclonal Antibody (Catalog # FAB2754A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: PIR-B

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Long Name
Paired-Ig-like Receptor B
Entrez Gene IDs
18733 (Mouse); 65146 (Rat)
Alternate Names
CD85a; HL9; ILT5; ILT-5; leukocyte immunoglobulin like receptor B3; LILRA6; LILRB3; LIR3; LIR-3; PIRB; PIR-B

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