Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 326414
View all PIR-B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
Gly24-Gly635
Accession # AAH26937

Specificity

Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse PIR‑B APC‑conjugated Antibody

Detection of PIR-B antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Anti-Mouse CD19 PE-conjugated Monoclonal Antibody and either (A) Rat Anti-Mouse PIR-B APC-conjugated Monoclonal Antibody (Catalog # FAB2754A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse PIR‑B APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Mouse splenocytes

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: PIR-B

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Long Name

Paired-Ig-like Receptor B

Alternate Names

LILRB3, PIRB

Entrez Gene IDs

18733 (Mouse); 65146 (Rat)

Gene Symbol

PIRB

UniProt

AAH26937 (Mouse)

Additional PIR-B Products

Product Documents for Mouse PIR‑B APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PIR‑B APC‑conjugated Antibody

For research use only

Related Research Areas

B Cell Activation

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