Mouse PIR-B Antibody (326414)

R&D Systems | Catalog # MAB2754

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 326414
View all PIR-B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
Gly24-Gly635
Accession # AAH26937

Specificity

Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse PIR-B Antibody (326414)

Detection of PIR-B by Western Blot

Detection of PIR-B by Western Blot

Pirb can be secreted via sEVs. A The growth curve of subcutaneous tumors. B The schematic diagram. C RFP fluorescence in GL261 cells in transwell plates. D RFP fluorescence in GL261 cells after incubation with CM from GL261-pirb cells with different treatments (MVs, sEVs and supernatant). E pirb/LILRB2, Alix and CD9 in U251-MVs, U251-sEVs, GL261-MVs and GL261-sEVs detected by WB. F pirb expression in sEVs from blood samples of the control mice and the tumor-bearing mice detected by WB. G LILRB2/pirb, Alix, and CD9 in U251-sEVs and GL261-sEVs detected by WB before or after GW4869 treatment. H The sEV amount and cell number detected by nanoflow cytometry and CCK8 assays. I pirb, Alix and CD9 in GL261-sEVs and GL261-pirb-sEVs were detected by WB. J The amount of sEVs in GL261 and GL261-pirb cells was detected by nanoflow cytometry. *P < 0.05, ** P < 0.01, ***P < 0.001, or ****P < 0.0001, ns indicates no statistical significance Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36853330), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of PIR-B by Western Blot

Detection of PIR-B by Western Blot

Pirb can be secreted via sEVs. A The growth curve of subcutaneous tumors. B The schematic diagram. C RFP fluorescence in GL261 cells in transwell plates. D RFP fluorescence in GL261 cells after incubation with CM from GL261-pirb cells with different treatments (MVs, sEVs and supernatant). E pirb/LILRB2, Alix and CD9 in U251-MVs, U251-sEVs, GL261-MVs and GL261-sEVs detected by WB. F pirb expression in sEVs from blood samples of the control mice and the tumor-bearing mice detected by WB. G LILRB2/pirb, Alix, and CD9 in U251-sEVs and GL261-sEVs detected by WB before or after GW4869 treatment. H The sEV amount and cell number detected by nanoflow cytometry and CCK8 assays. I pirb, Alix and CD9 in GL261-sEVs and GL261-pirb-sEVs were detected by WB. J The amount of sEVs in GL261 and GL261-pirb cells was detected by nanoflow cytometry. *P < 0.05, ** P < 0.01, ***P < 0.001, or ****P < 0.0001, ns indicates no statistical significance Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36853330), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse PIR-B Antibody (326414)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse PIR-B
under non-reducing conditions only

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PIR-B

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Long Name

Paired-Ig-like Receptor B

Alternate Names

LILRB3, PIRB

Entrez Gene IDs

18733 (Mouse); 65146 (Rat)

Gene Symbol

PIRB

UniProt

AAH26937 (Mouse)

Additional PIR-B Products

Product Documents for Mouse PIR-B Antibody (326414)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse PIR-B Antibody (326414)

For research use only

Related Research Areas

B Cell Activation

Citations for Mouse PIR-B Antibody (326414)

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