Mouse PIR-B Antibody

Catalog # Availability Size / Price Qty
MAB2754
MAB2754-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse PIR-B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.
Source
Monoclonal Rat IgG2A Clone # 326414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
Gly24-Gly635
Accession # AAH26937
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse PIR-B
under non-reducing conditions only
Flow Cytometry
2.5 µg/106 cells
Mouse splenocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIR-B

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Long Name
Paired-Ig-like Receptor B
Entrez Gene IDs
18733 (Mouse); 65146 (Rat)
Alternate Names
CD85a; HL9; ILT5; ILT-5; leukocyte immunoglobulin like receptor B3; LILRA6; LILRB3; LIR3; LIR-3; PIRB; PIR-B

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Citations for Mouse PIR-B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation.
    Authors: Williams, Jesse W, Tjota, Melissa, Clay, Bryan S, Vander Lugt, Bryan, Bandukwala, Hozefa S, Hrusch, Cara L, Decker, Donna C, Blaine, Kelly M, Fixsen, Bethany, Singh, Harinder, Sciammas, Roger, Sperling, Anne I
    Nat Commun, 2013;4(0):2990.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. SIRP{alpha}/CD172a Regulates Eosinophil Homeostasis.
    Authors: Verjan Garcia N, Umemoto E, Saito Y, Yamasaki M, Hata E, Matozaki T, Murakami M, Jung YJ, Woo SY, Seoh JY, Jang MH, Aozasa K, Miyasaka M
    J. Immunol., 2011;187(5):2268-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Classical major histocompatibility complex class I molecules in motoneurons: new actors at the neuromuscular junction.
    Authors: Thams S, Brodin P, Plantman S, Saxelin R, Karre K, Cullheim S
    J. Neurosci., 2009;29(43):13503-15.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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