Mouse PIR-B PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2754P

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Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (2)
FAQs
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Reviews (1)

Mouse PIR-B PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PIR-B in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse PIR‑A.
Source
Monoclonal Rat IgG2A Clone # 326414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PIR-B
Gly24-Gly635
Accession # AAH26937
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PIR-B antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of PIR‑B in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse PIR-B PE-conjugated Monoclonal Antibody (Catalog # FAB2754P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PIR-B

Mouse PIR-B is a 125 kDa type I transmembrane glycoprotein with six Ig-like domains in its extracellular domain (ECD) and four ITIM-like sequences in its cytoplasmic domain. The ECD of PIR-B is highly homologous to the ECDs of multiple mouse PIR-A receptors (92-99% amino acid sequence homology), which have short cytoplasmic tails lacking ITIM motifs. PIR-A receptors have a charged residue in their transmembrane domain that facilitates interaction with ITAM-containing adaptor molecules. Whereas PIR-A receptors deliver activation signals, PIR-B can inhibit receptor-mediated activation signaling. PIR-A and PIR-B have been shown to bind various mouse MHC class I molecules. They have been proposed to be orthologs of human leukocyte immunoglobulin-like receptors.

Long Name
Paired-Ig-like Receptor B
Entrez Gene IDs
18733 (Mouse); 65146 (Rat)
Alternate Names
CD85a; HL9; ILT5; ILT-5; leukocyte immunoglobulin like receptor B3; LILRA6; LILRB3; LIR3; LIR-3; PIRB; PIR-B

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Citations for Mouse PIR-B PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CAMKs support development of acute myeloid leukemia
    Authors: X Kang, C Cui, C Wang, G Wu, H Chen, Z Lu, X Chen, L Wang, J Huang, H Geng, M Zhao, Z Chen, M Müschen, HY Wang, CC Zhang
    J Hematol Oncol, 2018-02-27;11(1):30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Inhibitory receptors bind ANGPTLs and support blood stem cells and leukaemia development.
    Authors: Zheng J, Umikawa M, Cui C, Li J, Chen X, Zhang C, Hyunh H, Kang X, Silvany R, Wan X, Ye J, Canto AP, Chen SH, Wang HY, Ward ES, Zhang CC
    Nature, 2012-05-30;485(7400):656-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse PIR-B PE-conjugated Antibody
By Anonymous on 08/16/2017
Application: Flow Sample Tested: Splenocytes Species: Mouse

Naive C57Bl/6 splenocytes were stained with anti-PirB.