Mouse PlGF-2 Quantikine ELISA Kit

  (10 citations)     
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Product Details
Assay Procedure
Citations (10)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
  • Sensitivity
    1.84 pg/mL
  • Assay Range
    23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse PlGF-2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse PlGF-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PlGF-2 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse PlGF-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PlGF-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PlGF-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 58 136 470 63 155 493
Standard Deviation 4.6 7.6 14 6 15 43
CV% 7.9 5.6 3 9.5 9.7 8.7

Recovery

The recovery of mouse PlGF-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 97 84-119
EDTA Plasma (n=6) 94 88-104
Serum (n=6) 90 81-102
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse PlGF-2 in each matrix were diluted with Calibrator Diluent and then assayed.
 PlGF-2 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PlGF-2

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra-and inter-chain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.

In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF-2 has been identified. Mouse PlGF-2 encodes a 158 amino acid residue precursor protein. Mouse PlGF-2 shares 65% amino acid identity with human PlGF-2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14.

  • Long Name:
    Placenta Growth Factor 2
  • Entrez Gene IDs:
    5228 (Human); 18654 (Mouse)
  • Alternate Names:
    PlGF2; PlGF-2
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Sample Type
  1. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Type: Plasma
  2. Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.
    Authors: Oladipupo SS, Hu S, Santeford AC, Yao J, Kovalski JR, Shohet RV, Maslov K, Wang LV, Arbeit JM
    Blood, 2011;117(15):4142-53.
    Species: Human
    Sample Type: Plasma
  3. Placental growth factor mediates aldosterone-dependent vascular injury in mice.
    Authors: Jaffe IZ, Newfell BG, Aronovitz M
    J. Clin. Invest., 2010;120(11):3891-900.
    Species: Mouse
    Sample Type: Tissue Homogenates
  4. Inhibitory effect of atractylenolide I on angiogenesis in chronic inflammation in vivo and in vitro.
    Authors: Wang C, Duan H, He L
    Eur. J. Pharmacol., 2009;612(1):143-52.
    Species: Mouse
    Sample Type: Air Pouch Exudate
  5. alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
    Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
    Blood, 2007;111(4):1980-8.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  6. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
    Species: Mouse
    Sample Type: Plasma
  7. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Mouse
    Sample Type: Tissue Homogenates
  8. Impaired adipose tissue development in mice with inactivation of placental growth factor function.
    Authors: Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D
    Diabetes, 2006;55(10):2698-704.
    Species: Mouse
    Sample Type: Tissue Homogenates
  9. Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
    Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
    J. Immunol., 2005;174(1):215-22.
    Species: Mouse
    Sample Type: Serum
  10. The human herpes virus 8-encoded chemokine receptor is required for angioproliferation in a murine model of Kaposi's sarcoma.
    Authors: Jensen KK, Manfra DJ, Grisotto MG, Martin AP, Vassileva G, Kelley K, Schwartz TW, Lira SA
    J. Immunol., 2005;174(6):3686-94.
    Species: Mouse
    Sample Type: Tissue Homogenates

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