Mouse PlGF-2 Quantikine ELISA Kit

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MP200
Mouse PlGF-2 ELISA Standard Curve
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Product Details
Procedure
Citations (17)
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Mouse PlGF-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Sensitivity
1.84 pg/mL
Assay Range
23.4 - 1,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse PlGF-2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Mouse PlGF-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PlGF-2 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse PlGF-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PlGF-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PlGF-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 58 136 470 63 155 493
Standard Deviation 4.6 7.6 14 6 15 43
CV% 7.9 5.6 3 9.5 9.7 8.7

Recovery

The recovery of mouse PlGF-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 97 84-119
EDTA Plasma (n=6) 94 88-104
Serum (n=6) 90 81-102

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse PlGF-2 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse PlGF-2 ELISA Linearity

Scientific Data

Mouse PlGF-2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF-2

Placenta growth factor (PlGF) is a dimeric growth factor in the PDGF/VEGF family. Alternative splicing generates at least three human PlGF isoforms PlGF 1, PlGF 2, and PlGF 3. PlGF 2 is the only isoform that contains a heparin binding region. It is expressed by villous trophoblasts, decidual cells, erythroblasts, keratinocytes, and some endothelial cells. PlGF competes with VEGF for binding and activating VEGF R1/Flt 1. This results in increased angiogenesis by making more VEGF available for activation of VEGF R2. PlGF 2 also exhibits heparin dependent binding of Neuropilins-1 and 2 which serve as VEGF co-receptors. It can inhibit nerve growth cone collapse and induce monocyte production of inflammatory cytokines including VEGF.

Long Name:
Placenta Growth Factor 2
Entrez Gene IDs:
5228 (Human); 18654 (Mouse)
Alternate Names:
OORS; PlGF2; PlGF-2
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse PlGF-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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  1. Neutralization of the induced VEGF-A potentiates the therapeutic effect of an anti-VEGFR2 antibody on gastric cancer in vivo
    Authors: T Mashima, T Wakatsuki, N Kawata, MK Jang, A Nagamori, H Yoshida, K Nakamura, T Migita, H Seimiya, K Yamaguchi
    Scientific Reports, 2021;11(1):15125.
    Species: Mouse
    Sample Types: Plasma
  2. Acid Sensing Ion Channel 2a Is Reduced in the Reduced Uterine Perfusion Pressure Mouse Model and Increases Seizure Susceptibility in Pregnant Mice
    Authors: M Jones-Muha, Q Shao, L Cain-Shiel, JP Shaffery, JP Warrington
    Cells, 2021;10(5):.
    Species: Mouse
    Sample Types: Serum
  3. High-fat diet from parental generation exaggerates body and adipose tissue weights in pregnant offspring
    Authors: FT Spradley
    PLoS ONE, 2020;15(8):e0237708.
    Species: Rat
    Sample Types: Plasma
  4. High-fat diet from parental generation exaggerates body and adipose tissue weights in pregnant offspring
    Authors: FT Spradley
    PLoS ONE, 2020;15(8):e0237708.
    Species: Rat
    Sample Types: Plasma
  5. Adrenergic receptor blockade attenuates placental ischemia-induced hypertension
    Authors: FT Spradley, Y Ge, BP Haynes, JP Granger, CD Anderson
    Physiol Rep, 2018;6(17):e13814.
    Species: Rat
    Sample Types: Plasma
  6. Induction of hemangiosarcoma in mice after chronic treatment with S1P-modulator siponimod and its lack of relevance to rat and human
    Authors: F Pognan, JA Mahl, M Papoutsi, D Ledieu, M Raccuglia, D Theil, SB Voytek, PJ Devine, K Kubek-Luck, N Claudio, A Cordier, A Heier, C Kolly, A Hartmann, SD Chibout, P Bouchard, C Trendelenb
    Arch. Toxicol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Blockade of placental growth factor reduces vaso-occlusive complications in murine models of sickle cell disease
    Authors: JM Gu, S Yuan, D Sim, K Abe, P Liu, M Rosenbruch, P Bringmann, K Kauser
    Exp. Hematol., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  8. Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors
    Authors: M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos
    Mol. Cancer Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Plasma
  9. Conditional HIF-1 induction produces multistage neovascularization with stage-specific sensitivity to VEGFR inhibitors and myeloid cell independence.
    Authors: Oladipupo SS, Hu S, Santeford AC, Yao J, Kovalski JR, Shohet RV, Maslov K, Wang LV, Arbeit JM
    Blood, 2011;117(15):4142-53.
    Species: Human
    Sample Types: Plasma
  10. Placental growth factor mediates aldosterone-dependent vascular injury in mice.
    Authors: Jaffe IZ, Newfell BG, Aronovitz M
    J. Clin. Invest., 2010;120(11):3891-900.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Inhibitory effect of atractylenolide I on angiogenesis in chronic inflammation in vivo and in vitro.
    Authors: Wang C, Duan H, He L
    Eur. J. Pharmacol., 2009;612(1):143-52.
    Species: Mouse
    Sample Types: Air Pouch Exudate
  12. Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy.
    Authors: Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(43):17069-74.
    Species: Mouse
    Sample Types: Plasma
  13. alpha2beta1 integrin expression in the tumor microenvironment enhances tumor angiogenesis in a tumor cell-specific manner.
    Authors: Zhang Z, Ramirez NE, Yankeelov TE, Li Z, Ford LE, Qi Y, Pozzi A, Zutter MM
    Blood, 2007;111(4):1980-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Impaired adipose tissue development in mice with inactivation of placental growth factor function.
    Authors: Lijnen HR, Christiaens V, Scroyen I, Voros G, Tjwa M, Carmeliet P, Collen D
    Diabetes, 2006;55(10):2698-704.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Placenta growth factor in diabetic wound healing: altered expression and therapeutic potential.
    Authors: Cianfarani F, Zambruno G, Brogelli L, Sera F, Lacal PM, Pesce M, Capogrossi MC, Failla CM, Napolitano M, Odorisio T
    Am. J. Pathol., 2006;169(4):1167-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
    Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
    J. Immunol., 2005;174(1):215-22.
    Species: Mouse
    Sample Types: Serum
  17. The human herpes virus 8-encoded chemokine receptor is required for angioproliferation in a murine model of Kaposi's sarcoma.
    Authors: Jensen KK, Manfra DJ, Grisotto MG, Martin AP, Vassileva G, Kelley K, Schwartz TW, Lira SA
    J. Immunol., 2005;174(6):3686-94.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Mouse PlGF-2 Quantikine ELISA Kit
By Anonymous on 12/11/2021
Sample Tested: Serum