Mouse PlGF-2 Quantikine ELISA Kit

R&D Systems | Catalog # MP200

R&D Systems
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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL)

Sensitivity

1.84 pg/mL

Assay Range

23.4-1500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
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Product Summary for Mouse PlGF-2 Quantikine ELISA Kit

The Quantikine Mouse PlGF-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse PlGF-2 levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant mouse PlGF-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse PlGF-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse PlGF-2.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Mouse

Specificity

Natural and recombinant mouse PlGF-2

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 58 136 470 63 155 493
Standard Deviation 4.6 7.6 14 6.0 15 43
CV% 7.9 5.6 3.0 9.5 9.7 8.7

Recovery for Mouse PlGF-2 Quantikine ELISA Kit

The recovery of mouse PlGF-2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 97 84-119
EDTA Plasma (n=6) 94 88-104
Serum (n=6) 90 81-102

Linearity

To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse PlGF-2 in each matrix were diluted with Calibrator Diluent and then assayed.

Mouse PlGF-2 ELISA Linearity

Scientific Data Images for Mouse PlGF-2 Quantikine ELISA Kit

Mouse PlGF-2 ELISA Standard Curve

Mouse PlGF-2 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PlGF-2

Placenta growth factor (PlGF) is a dimeric growth factor in the PDGF/VEGF family. Alternative splicing generates at least three human PlGF isoforms PlGF-1, PlGF-2, and PlGF-3. PlGF-2 is the only isoform that contains a heparin binding region. It is expressed by villous trophoblasts, decidual cells, erythroblasts, keratinocytes, and some endothelial cells. PlGF competes with VEGF for binding and activating VEGF R1/Flt-1. This results in increased angiogenesis by making more VEGF available for activation of VEGF R2. PlGF-2 also exhibits heparin dependent binding of Neuropilins-1 and -2 which serve as VEGF co-receptors. It can inhibit nerve growth cone collapse and induce monocyte production of inflammatory cytokines including VEGF.

Long Name

Placenta Growth Factor 2

Alternate Names

PlGF2

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol

PGF

Additional PlGF-2 Products

Product Documents for Mouse PlGF-2 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse PlGF-2 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse PlGF-2 Quantikine ELISA Kit

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Protocols

View specific protocols for Mouse PlGF-2 Quantikine ELISA Kit (MP200):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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