Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 192703
View all Podocalyxin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4

Specificity

Detects mouse Podocalyxin in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Podocalyxin APC‑conjugated Antibody

Detection of Podocalyxin antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Podocalyxin in D3 Mouse Cell Line by Flow Cytometry.

Undifferentiated D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Podocalyxin APC‑conjugated Monoclonal Antibody (Catalog # FAB1556A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Podocalyxin in bEND-3 cells by Flow Cytometry.

bEND-3 cells were stained with Rat Anti-Mouse Podocalyxin APC‑conjugated Monoclonal Antibody (Catalog # FAB1556A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Podocalyxin APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Undifferentiated D3 mouse embryonic stem cell line and bEND-3 cells.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References

  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Entrez Gene IDs

5420 (Human); 27205 (Mouse)

Gene Symbol

PODXL

UniProt

Q9R0M4

Additional Podocalyxin Products

Product Documents for Mouse Podocalyxin APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Podocalyxin APC‑conjugated Antibody

For research use only

Citations for Mouse Podocalyxin APC‑conjugated Antibody

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