Mouse Podocalyxin Antibody

Catalog # Availability Size / Price Qty
MAB1556
MAB1556-SP
Mouse Podocalyxin Antibody in Immunocytochemistry
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews

Mouse Podocalyxin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 192703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Podocalyxin
Flow Cytometry
2.5 µg/106 cells
D3 mouse embryonic stem cell line
Immunohistochemistry
1-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Podocalyxin in bEnd.3 Mouse Cell Line. View Larger

Podocalyxin in bEnd.3 Mouse Cell Line. Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Podocalyxin in Mouse Kidney Tissue. View Larger

Podocalyxin in Mouse Kidney Tissue. Podocalyxin was detected in perfusion fixed frozen sections of mouse kidney tissue using Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) at 1.7 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

Product Datasheets

Citations for Mouse Podocalyxin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Sonic hedgehog connects podocyte injury to mesangial activation and glomerulosclerosis
    Authors: D Zhou, H Fu, Y Han, L Zhang, S Liu, L Lin, DB Stolz, Y Liu
    JCI Insight, 2019;4(22):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Sequential formation and resolution of multiple rosettes drive embryo remodelling after implantation
    Authors: N Christodou, C Kyprianou, A Weberling, R Wang, G Cui, G Peng, N Jing, M Zernicka-G
    Nat. Cell Biol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development
    Authors: N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi
    Dev. Cell, 2017;41(4):349-365.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition
    Authors: Katsumi Fumoto
    Development, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. In vivo RNA interference models of inducible and reversible Sirt1 knockdown in kidney cells.
    Authors: Chuang P, Xu J, Dai Y, Jia F, Mallipattu S, Yacoub R, Gu L, Premsrirut P, He J
    Am J Pathol, 2014;184(7):1940-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Podocalyxin selectively marks erythroid-committed progenitors during anemic stress but is dispensable for efficient recovery.
    Authors: Maltby S, Hughes MR, Zbytnuik L, Paulson RF, McNagny KM
    Exp. Hematol., 2009;37(1):10-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. A new mouse model of immune-mediated podocyte injury.
    Authors: Meyer TN, Schwesinger C, Wahlefeld J, Dehde S, Kerjaschki D, Becker JU, Stahl RA, Thaiss F
    Kidney Int., 2007;72(7):841-52.
    Species: Mouse
    Sample Types: Urine
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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