Mouse Podocalyxin Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Podocalyxin in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2B Clone # 192703
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
    Ser21-Arg402
    Accession # Q9R0M4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse Podocalyxin
  • Flow Cytometry
    2.5 µg/106 cells
    D3 mouse embryonic stem cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Podocalyxin in bEnd.3 Mouse Cell Line. Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Podocalyxin
Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).
  • References:
    1. Li, J. et al. (2001) DNA Seq. 12(5):407.
    2. Hara, T. et al. (1999) Immunity 11(5):567.
  • Entrez Gene IDs:
    5420 (Human); 27205 (Mouse)
  • Alternate Names:
    GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. In vivo RNA interference models of inducible and reversible Sirt1 knockdown in kidney cells.
    Authors: Chuang P, Xu J, Dai Y, Jia F, Mallipattu S, Yacoub R, Gu L, Premsrirut P, He J
    Am J Pathol, 2014;184(7):1940-56.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Podocalyxin selectively marks erythroid-committed progenitors during anemic stress but is dispensable for efficient recovery.
    Authors: Maltby S, Hughes MR, Zbytnuik L, Paulson RF, McNagny KM
    Exp. Hematol., 2009;37(1):10-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. A new mouse model of immune-mediated podocyte injury.
    Authors: Meyer TN, Schwesinger C, Wahlefeld J, Dehde S, Kerjaschki D, Becker JU, Stahl RA, Thaiss F
    Kidney Int., 2007;72(7):841-52.
    Species: Mouse
    Sample Type: Urine
    Application: WB
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