Mouse Podocan Biotinylated Antibody

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Mouse Podocan Biotinylated Antibody Summary

Species Reactivity
Detects mouse Podocan in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) Podocan is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Podocan
Accession # Q7TQ62
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse Podocan (Catalog # 3104-PO)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Podocan

Mouse Podocan is a 95 kDa, secreted glycoprotein that is a class V member of the small leucine rich protein gene family (1, 2). It is synthesized as a 611 amino acid (aa) precursor that contains a 23 aa signal sequence, a cysteine-rich region, a series of leucine rich repeats (LRRs), and an extensive acidic C-terminal domain (4, 5). The 15 aa cysteine-rich region (aa 69-84) shows a CX3CXCX7C motif, qualifying it as a class V SLRP family member. This is followed by twenty LRRs, thirteen of which are type T (4xLeu; 1xPhe) and seven type S (4xLeu; 2xPro). The LRRs run uninterrupted from aa 89-559. The C-terminal seventeen amino acids contain fourteen Glu residues. The negative charge associated with these residues may play a role in basement membrane permeability (4). Mature mouse Podocan is 93% and 94% aa identical to human and canine Podocan, respectively. Over the last 504 aa, mouse Podocan shares 98% aa sequence identity with rat Podocan. Podocan is apparently secreted by podocytes and vascular endothelial cells, and deposited in the underlying basement membrane (4). Podocan is known to bind to type I collagen, and have an inhibitory effect on the migration of Podocan-transfected CHO cells (5). The significance of this is unclear.

  1. Matsushima, N. et al. (2000) Proteins 38:210.
  2. Matsushima, N. et al. (2004) Proteins 54:394.
  3. Ross, M.D. et al. (2005) Cell. Mol. Life Sci. 62:2771.
  4. Ross, M.D. et al. (2003) J. Biol. Chem. 278:33248.
  5. Shimizu-Hirota, R. et al. (2004) FEBS Lett. 563:69.
Entrez Gene IDs
127435 (Human); 242608 (Mouse)
Alternate Names
PCAN; PODN; podocan proteoglycan; Podocan; SLRR5A; SLRR5AMGC24995

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