Mouse Prostasin/Prss8 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2968
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Mouse Prostasin/Prss8 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Prostasin/Prss8 in ELISAs and Western blots.  In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human Prostasin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Prostasin/Prss8
Ala30-Gln289
Accession # Q99L44
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Prostasin/Prss8 (Catalog # 2968-SE)

Mouse Prostasin/Prss8 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Prostasin/Prss8 Antibody (Catalog # AF2968)

Standard: Recombinant Mouse Prostasin/Prss8 Protein, CF (Catalog # 2968-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prostasin/Prss8

Prostasin encoded by the Prss8 gene, also known as channel activating protease 1, is a serine protease with a trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of prostasin is involved in the regulation of epithelial sodium channels (4, 5). Secreted from NS0 cells as the active form, the purified recombinant mouse (rm) Prostasin most likely consists of a disulfide bond-linked two chains with N-terminal sequences of I45TGGGSAKPG and A30DGTE, respectively (3). The enzymatic activity of rmProstasin is effectively inhibited by rmHAI-1 and rmHAI‑2B (Catalog # 1141-PI and 1107-PI).

References
  1. Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
  2. Yu J.X. et al. (1994) J. Biol. Chem. 269:18843.
  3. Yu J.X. et al. (1995) J. Biol. Chem. 270:13483.
  4. Caldwell R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C190.
  5. Tong Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.
Entrez Gene IDs
5652 (Human); 76560 (Mouse)
Alternate Names
CAP1; channel-activating protease 1; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.120; Prostasin; protease, serine, 8; Prss8; Serine protease 8

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