Mouse Protocadherin-12 Alexa Fluor® 750-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Protocadherin-12

PCDH12 (Protocadherin 12; also VE‑Cadherin 2) is a 150‑160 kDa glycoprotein member of the PCDH family of molecules. It exhibits limited expression, being found on endothelial cells, renal mesangium, Sertoli cells, and glycogen‑positive trophoblast cells. PCDH is a transmembrane protein that forms homotypic aggregates in a calcium‑dependent manner. Although this would suggest a prominent role in cell-cell adhesion, it is unclear if this is the only activity for the molecule. In any case, it appears that PCDH12 does play an important role in placental development. Mature mouse PCDH12 is an 1163 amino acid (aa) type I transmembrane protein (aa 18‑1180). It possesses a 699 aa extracellular region (aa 18‑716) that contains six cadherin domains (aa 28‑711), plus a 21 aa transmembrane segment coupled to a lengthy 443 aa cytoplasmic domain (aa 738‑1180). Based on human, mouse PCDH12 will undergo proteolytic processing. MMP activity will first generate a 90 kDa circulating extracellular fragment and a 60 kDa membrane‑bound fragment, followed by gamma ‑secretase which has the potential to further act on the membrane‑bound fragment, generating a cytosolic 50 kDa fragment. There is one potential alternative start site at Met1060. Over aa 18‑716, mouse PCDH12 shares 95% and 82% aa sequence identity with rat and human PCDH12, respectively.

Product Datasheets

Related Research Areas

• Cadherin Superfamily
• Calcium-binding Proteins and Related Molecules