Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all Protogenin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Protogenin
Val24-Thr943
Accession # Q2EY15

Specificity

Detects mouse and human Protogenin in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Mouse Protogenin Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Protogenin

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protogenin

Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively.

Alternate Names

IGDCC5, PRTG, RPTG, Shen-Dan

Entrez Gene IDs

283659 (Human); 235472 (Mouse); 315806 (Rat)

Gene Symbol

PRTG

UniProt

Q2EY15

Additional Protogenin Products

Product Documents for Mouse Protogenin Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Protogenin Biotinylated Antibody

For research use only

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