Mouse R-Spondin 4 Antibody

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Mouse R-Spondin 4 Antibody Summary

Species Reactivity
Detects mouse R‑Spondin 4 in direct ELISAs and Western blots. In Western blots, 50‑100% cross‑reactivity with recombinant human (rh) R‑Spondin 4 is observed and no cross-reactivity with recombinant mouse (rm) R‑Spondin 1, rmR‑Spondin 3, or rhR‑Spondin 2 is observed.
Monoclonal Rat IgG2B Clone # 435203
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse R‑Spondin 4
Accession # Q8BJ73
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse R-Spondin 4

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 4

R-Spondin 4 (RSPO4, roof plate-specific spondin 4), also called cysteine-rich and single thrombospondin domain containing-4 (Cristin 4), is an ~33 kDa secreted heparin-binding protein that shares ~35% amino acid (aa) identity with three other R-Spondin family members (1-3). All are positive modulators of Wnt/ beta -catenin signaling, but R-Spondin 4 may be somewhat weaker than other R-Spondins (2). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK‑1‑mediated internalization (1, 4). Like other R‑Spondins, mouse R-Spondin 4 (234 aa) contains a signal sequence (aa 1-19), two adjacent cysteine-rich furin-like domains (aa 85-128) with one potential tyrosine phosphorylation site (aa 114), followed by a thrombospondin (TSP-1) motif (aa 137‑197) and a region rich in basic residues (aa 199‑234). The furin-like domains are sufficient for beta -catenin stabilization (2). Mature mouse R‑Spondin 4 shares 81%, 97%, 79%, 77% and 76% aa identity with human, rat, bovine, equine and canine R-Spondin 4, respectively. There is one potential isoform where Arg substitutes for the C‑terminal 82 amino acids (5). Each R‑Spondin has a distinct expression pattern (6). In the mouse, R‑Spondin 4 mRNA is found during development of limb bud mesenchyme, nail beds, heart and teeth (6‑8). In humans, mutations of R‑Spondin 4 have been found to cause anonychia, a condition in which fingernails and toenails are absent (8‑10).

  1. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  5. Entrez Accession # Q8BJ73, Isoform 2.
  6. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  7. Pemberton, T.J. et al. (2007) Dev. Dyn. 236:2245.
  8. Ishii, Y. et al. (2008) J. Invest. Dermatol. 128:867.
  9. Blaydon, D.C. et al. (2006) Nat. Genet. 38:1245.
  10. Bergmann, C. et al. (2006) Am. J. Hum. Genet. 79:1105.
Long Name
Roof Plate-specific Spondin 4
Entrez Gene IDs
343637 (Human); 228770 (Mouse)
Alternate Names
C20orf182; chromosome 20 open reading frame 182; Cristin 4; CRISTIN4; dJ824F16.3; FLJ16018; hRspo4; Roof plate-specific spondin-4; RSPO4; RSpondin 4; R-Spondin 4; R-spondin family, member 4; R-spondin-4

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