Mouse Rae-1 Pan Specific APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB17582A

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Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (8)
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Mouse Rae-1 Pan Specific APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
Source
Monoclonal Rat IgG2A Clone # 186107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
Leu29-Ser227
Accession # Q9JI58
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Rae-1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific APC-conjugated Monoclonal Antibody (Catalog # FAB17582A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1; Rae1; Rae-1

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Citations for Mouse Rae-1 Pan Specific APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. NKG2D is Required for Regulation of Lung Pathology and Dendritic Cell Function Following RSV Infection
    Authors: H Liu, AR Osterburg, J Flury, S Huang, FX McCormack, SA Cormier, MT Borchers
    J. Infect. Dis., 2018-10-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Visualizing the Rapid and Dynamic Elimination of Allogeneic T Cells in Secondary Lymphoid Organs
    Authors: Y Kanda, A Takeuchi, M Ozawa, Y Kurosawa, T Kawamura, D Bogdanova, H Iioka, E Kondo, Y Kitazawa, H Ueta, K Matsuno, T Kinashi, T Katakai
    J. Immunol., 2018-06-20;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The p36 Isoform of Murine Cytomegalovirus m152 Protein Suffices for Mediating Innate and Adaptive Immune Evasion
    Authors: Annette Fink, Angeliqué Renzaho, Matthias J. Reddehase, Niels A. W. Lemmermann
    Viruses
  4. A transplantable TH-MYCN transgenic tumor model in C57Bl/6 mice for preclinical immunological studies in neuroblastoma.
    Authors: Kroesen M, Nierkens S, Ansems M, Wassink M, Orentas R, Boon L, den Brok M, Hoogerbrugge P, Adema G
    Int J Cancer, 2013-09-14;134(6):1335-45.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. NKG2D CAR T-cell therapy inhibits the growth of NKG2D ligand heterogeneous tumors.
    Authors: Spear P, Barber A, Rynda-Apple A, Sentman C
    Immunol Cell Biol, 2013-04-30;91(6):435-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  6. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012-06-29;189(3):1162-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Genomic modeling of tumor onset and progression in a mouse model of aggressive human liver cancer
    Authors: Cédric Coulouarn, Valentina M. Factor, Elizabeth A. Conner, Snorri S. Thorgeirsson
    Carcinogenesis
  8. NK cell regulation of CD4 T cell-mediated graft-versus-host disease.
    Authors: Rivas MN, Hazzan M, Weatherly K, Gaudray F, Salmon I, Braun MY
    J. Immunol., 2010-05-19;184(12):6790-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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