Mouse Rae-1 Pan Specific Antibody

(16 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
  • Source
    Monoclonal Rat IgG2A Clone # 186107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
    Leu29-Ser227
    Accession # Q9JI58
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae‑1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rae-1
Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.
  • Long Name:
    Retinoic Acid Early Transcript 1
  • Entrez Gene IDs:
    8480 (Human); 19368 (Mouse)
  • Alternate Names:
    Rae1; Rae-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo
    J Immunol, 2016;196(11):4805-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. A Herpesviral induction of RAE-1 NKG2D ligand expression occurs through release of HDAC mediated repression
    Elife, 2016;5(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015;135(5):1311-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
    Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
    PLoS ONE, 2015;10(12):e0143224.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Engagement of NKG2D on bystander memory CD8 T cells promotes increased immunopathology following Leishmania major infection.
    Authors: Crosby E, Goldschmidt M, Wherry E, Scott P
    PLoS Pathog, 2014;10(2):e1003970.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. The E3 ligase Cbl-b and TAM receptors regulate cancer metastasis via natural killer cells.
    Authors: Paolino, Magdalen, Choidas, Axel, Wallner, Stephani, Pranjic, Blanka, Uribesalgo, Iris, Loeser, Stefanie, Jamieson, Amanda M, Langdon, Wallace, Ikeda, Fumiyo, Fededa, Juan Pab, Cronin, Shane J, Nitsch, Roberto, Schultz-Fademrecht, Carsten, Eickhoff, Jan, Menninger, Sascha, Unger, Anke, Torka, Robert, Gruber, Thomas, Hinterleitner, Reinhard, Baier, Gottfrie, Wolf, Dominik, Ullrich, Axel, Klebl, Bert M, Penninger, Josef M
    Nature, 2014;507(7493):508-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells.
    Authors: Tu M, Mahmoud A, Wight A, Mottashed A, Belanger S, Rahim M, Abou-Samra E, Makrigiannis A
    Cancer Res, 2014;74(14):3684-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. Activating receptor NKG2D targets RAE-1-expressing allogeneic neural precursor cells in a viral model of multiple sclerosis.
    Authors: Weinger J, Plaisted W, Maciejewski S, Lanier L, Walsh C, Lane T
    Stem Cells, 2014;32(10):2690-701.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. RAE-1 ligands for the NKG2D receptor are regulated by E2F transcription factors, which control cell cycle entry.
    Authors: Jung, Heiyoun, Hsiung, Benjamin, Pestal, Kathleen, Procyk, Emily, Raulet, David H
    J Exp Med, 2012;209(13):2409-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease.
    Authors: Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M
    J. Clin. Invest., 2009;119(3):636-49.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Intrinsic sensor of oncogenic transformation induces a signal for innate immunosurveillance.
    Authors: Unni AM, Bondar T, Medzhitov R
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(5):1686-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. Chimeric NKG2D receptor-bearing T cells as immunotherapy for ovarian cancer.
    Authors: Barber A, Zhang T, DeMars LR, Conejo-Garcia J, Roby KF, Sentman CL
    Cancer Res., 2007;67(10):5003-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. Blockade of NKG2D on NKT cells prevents hepatitis and the acute immune response to hepatitis B virus.
    Authors: Vilarinho S, Ogasawara K, Nishimura S, Lanier LL, Baron JL
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(46):18187-92.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. A role for activation-induced cytidine deaminase in the host response against a transforming retrovirus.
    Authors: Gourzi P, Leonova T, Papavasiliou FN
    Immunity, 2006;24(6):779-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. BCR/ABL promotes dendritic cell-mediated natural killer cell activation.
    Authors: Terme M, Borg C, Guilhot F, Masurier C, Flament C, Wagner EF, Caillat-Zucman S, Bernheim A, Turhan AG, Caignard A, Zitvogel L
    Cancer Res., 2005;65(14):6409-17.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  16. Chronic In Vivo Interaction of Dendritic Cells Expressing the Ligand Rae-1epsilon with NK Cells Impacts NKG2D Expression and Function.
    Authors: Morvan M, Champsaur M, Reizis B, Lanier L
    Immunohorizons, 0;1(3):10-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 16 Citations
Isotype Controls
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Rat IgG2A Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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