Mouse Rae-1 Pan Specific Antibody (186107)

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific Monoclonal Antibody (Catalog # MAB17582, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Detection of Rae-1 Pan Specific by Flow Cytometry

NK cell mediated non-self or induced-self recognition does not impact the expansion of BCR-ABL1+ cells.(A) The bar graph shows the mean absolute number (±SD) of B6 Dd (H-2bDd, CD45.1)-derived myeloid cells expressing BCR-ABL1 (GFP) in B6 (H-2b, CD45.2) recipients at day 8 after transplantation (MHC-I different, non-self recognition). (B) The expression of Rae-1 NKG2D ligands was determined on B6 (left) or BALB.B (right)-derived myeloid cells (CD11b+) expressing BCR-ABL1 on day 8 after transplantation into B6 mice (gray fill). Open histograms depict background staining on BM-derived CD11b+ cells of normal B6 mice. (C) The bar graph shows the mean absolute number (±SD) of BABL.B (H-2b, CD45.2)-derived myeloid cells expressing GFP (either control or BCR-ABL1) in B6 (H-2b, CD45.1) recipients at day 8 after transplantation (MHC-I matched, unrelated, induced-self recognition). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/22132120), licensed under a CC-BY license. Not internally tested by R&D Systems.