Mouse Rae-1 Pan Specific PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB17582P-100UG

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Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (9)
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Mouse Rae-1 Pan Specific PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
Source
Monoclonal Rat IgG2A Clone # 186107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
Leu29-Ser227
Accession # Q9JI58
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Rae‑1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 Pan Specific PE-conjugated Monoclonal Antibody (Catalog # FAB17582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Rae-1

Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Long Name
Retinoic Acid Early Transcript 1
Entrez Gene IDs
8480 (Human); 19368 (Mouse)
Alternate Names
Rae1; Rae-1

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Citations for Mouse Rae-1 Pan Specific PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity
    Authors: GK Griffin, J Wu, A Iracheta-V, JC Patti, J Hsu, T Davis, D Dele-Oni, PP Du, AG Halawi, JJ Ishizuka, SY Kim, S Klaeger, NH Knudsen, BC Miller, TH Nguyen, KE Olander, M Papanastas, S Rachimi, EJ Robitschek, EM Schneider, MD Yeary, MD Zimmer, JD Jaffe, SA Carr, JG Doench, WN Haining, KB Yates, RT Manguso, BE Bernstein
    Nature, 2021;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
    Authors: G Itakura, M Ozaki, N Nagoshi, S Kawabata, Y Nishiyama, K Sugai, T Iida, R Kashiwagi, T Ookubo, K Yastake, K Matsubayas, J Kohyama, A Iwanami, M Matsumoto, M Nakamura, H Okano
    Sci Rep, 2017;7(1):12996.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation.
    Authors: Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F
    Nat Neurosci, 2016;19(2):243-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Proinflammatory Proteins S100A8/S100A9 Activate NK Cells via Interaction with RAGE.
    Authors: Narumi K, Miyakawa R, Ueda R, Hashimoto H, Yamamoto Y, Yoshida T, Aoki K
    J Immunol, 2015;194(11):5539-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.
    Authors: Zhan R, Han Q, Zhang C, Tian Z, Zhang J
    Infect Immun, 2015;83(4):1641-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012;8(1):e1002481.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010;83(3):404-14.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis.
    Authors: Walsh KB, Lanier LL, Lane TE
    J. Virol., 2007;82(6):3031-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. NKG2D blockade prevents autoimmune diabetes in NOD mice.
    Authors: Ogasawara K, Hamerman JA, Ehrlich LR, Bour-Jordan H, Santamaria P, Bluestone JA, Lanier LL
    Immunity, 2004;20(6):757-67.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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