Mouse Rae-1 Pan Specific PE-conjugated Antibody

(7 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Rae‑1. It recognizes Rae-1 alpha, beta, δ, gamma and epsilon.
  • Source
    Monoclonal Rat IgG2A Clone # 186107
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
    Leu29-Ser227
    Accession # Q9JI58
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rae‑1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae‑1 Pan Specific PE‑conjugated Monoclonal Antibody (Catalog # FAB17582P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Rae-1
Rae-1 alpha, beta, gamma, δ and epsilon comprise a family of closely related (88-95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.
  • Long Name:
    Retinoic Acid Early Transcript 1
  • Entrez Gene IDs:
    8480 (Human); 19368 (Mouse)
  • Alternate Names:
    Rae1; Rae-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation.
    Authors: Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F
    Nat Neurosci, 2016;19(2):243-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Toll-Like receptor 2 (TLR2) and TLR9 play opposing roles in host innate immunity against Salmonella enterica serovar Typhimurium infection.
    Authors: Zhan R, Han Q, Zhang C, Tian Z, Zhang J
    Infect Immun, 2015;83(4):1641-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Proinflammatory Proteins S100A8/S100A9 Activate NK Cells via Interaction with RAGE.
    Authors: Narumi K, Miyakawa R, Ueda R, Hashimoto H, Yamamoto Y, Yoshida T, Aoki K
    J Immunol, 2015;194(11):5539-48.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
    Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL
    PLoS Pathog., 2012;8(1):e1002481.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. Murine trophoblast cells induce NK cell interferon-gamma production through KLRK1.
    Authors: Carayannopoulos LN, Barks JL, Yokoyama WM
    Biol. Reprod., 2010;83(3):404-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis.
    Authors: Walsh KB, Lanier LL, Lane TE
    J. Virol., 2007;82(6):3031-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. NKG2D blockade prevents autoimmune diabetes in NOD mice.
    Authors: Ogasawara K, Hamerman JA, Ehrlich LR, Bour-Jordan H, Santamaria P, Bluestone JA, Lanier LL
    Immunity, 2004;20(6):757-67.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PE-conjugated Antibody

Ctrl IC006P 16  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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