Detection of GABAA R beta 3 C-Terminus by Western Blot.
Western blot of the ~50 - 56 kDa beta 3 subunit of the GABAA R protein in rat brain lysate (control). Immunolabeling was absent in a brain lysate prepared from animals in which the beta 3 subunit had been knocked out (K/O).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: GABA-A R beta 3
GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat beta 3 isoform is a 58 kDa, 448 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 3 subunit is known to be phosphorylated on a consensus phosphorylation site (S408 of the precursor) that exists in the cytoplasmic domain between transmembrane segments 3 and 4. In contrast to beta 1 subunit modulation, PKC beta II-induced phosphorylation appears to increase GABAA receptor activity. PKA phosphorylation on both S408 and S409 also appears to increase receptor activity.
Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
Brandon, N.J. et al. (2003) Mol. Cell. Neurosci. 22:87.
McDonald, B.J. et al. (1998) Nat. Neurosci. 1:23.
gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
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