Home / GABA-A R beta 3 / Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

Catalog #: PPS032
1 Citation Datasheet / COA / SDS

Discontinued Product

PPS032 has been discontinued.
View all GABA-A R beta 3 products.
Detection of GABAAR beta 3 C-Terminus by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat GABA-A R beta 3 C-Terminus Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Mouse and rat ~50 - 56 kDa GABAA R beta 3 subunit, C-Terminus
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Fusion protein from the cytosolic loop of GABAA R beta 3 subunit, C-Terminus
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of GABA<sub>A</sub>R beta 3 C-Terminus antibody by Western Blot. View Larger

Detection of GABAAR beta 3 C-Terminus by Western Blot. Western blot of the ~50 - 56 kDa beta 3 subunit of the GABAAR protein in rat brain lysate (control). Immunolabeling was absent in a brain lysate prepared from animals in which the beta 3 subunit had been knocked out (K/O).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R beta 3

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat beta 3 isoform is a 58 kDa, 448 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 3 subunit is known to be phosphorylated on a consensus phosphorylation site (S408 of the precursor) that exists in the cytoplasmic domain between transmembrane segments 3 and 4. In contrast to beta 1 subunit modulation, PKC beta II-induced phosphorylation appears to increase GABAA receptor activity. PKA phosphorylation on both S408 and S409 also appears to increase receptor activity.

References
  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
  4. Brandon, N.J. et al. (2003) Mol. Cell. Neurosci. 22:87.
  5. McDonald, B.J. et al. (1998) Nat. Neurosci. 1:23.
Long Name
gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide
Entrez Gene IDs
2562 (Human); 14402 (Mouse); 24922 (Rat)
Alternate Names
ECA5; GABA A R beta 3; GABA(A) receptor beta-3 subunit; GABA(A) receptor subunit beta-3; GABAA receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit; GABAAR beta 3; GABAARb3; GABRB3; gamma-aminobutyric acid (GABA) A receptor, beta 3; gamma-aminobutyric acid receptor subunit beta-3; MGC9051

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Sub-chronic Exposure to Morphine Alters General Anesthetic Potency by Differentially Regulating the Expression of Neurotransmitter Receptor Subunits in Mice
    Authors: Y Chen, M Ou, X Hao, P Liang, Y Liang, Y Wang, Y Li, C Zhou
    Brain research bulletin, 2021-01-20;169(0):136-144.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

There are currently no reviews for this product. Be the first to review Mouse/Rat GABA-A R beta 3 C-Terminus Antibody and earn rewards!

Have you used Mouse/Rat GABA-A R beta 3 C-Terminus Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review