Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

R&D Systems | Catalog # PPS032

R&D Systems
Discontinued Product
PPS032 has been discontinued. An alternative/replacement product is available: NB300-199. View all GABA-A R beta 3 products.

Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GABA-A R beta 3 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytosolic loop of GABAA R beta 3 subunit, C-Terminus

Specificity

Mouse and rat ~50 - 56 kDa GABAA R beta 3 subunit, C-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

Detection of GABAAR beta 3 C-Terminus antibody by Western Blot.

Detection of GABAAR beta 3 C-Terminus by Western Blot.

Western blot of the ~50 - 56 kDa beta 3 subunit of the GABAAR protein in rat brain lysate (control). Immunolabeling was absent in a brain lysate prepared from animals in which the beta 3 subunit had been knocked out (K/O).

Applications for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R beta 3

GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat beta 3 isoform is a 58 kDa, 448 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 3 subunit is known to be phosphorylated on a consensus phosphorylation site (S408 of the precursor) that exists in the cytoplasmic domain between transmembrane segments 3 and 4. In contrast to beta 1 subunit modulation, PKC beta II-induced phosphorylation appears to increase GABAA receptor activity. PKA phosphorylation on both S408 and S409 also appears to increase receptor activity.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
  4. Brandon, N.J. et al. (2003) Mol. Cell. Neurosci. 22:87.
  5. McDonald, B.J. et al. (1998) Nat. Neurosci. 1:23.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide

Alternate Names

GABAAR beta 3, GABAARb3, GABRB3

Entrez Gene IDs

2562 (Human); 14402 (Mouse); 24922 (Rat)

Gene Symbol

GABRB3

Additional GABA-A R beta 3 Products

Product Documents for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

For research use only

Related Research Areas

GABA Receptors

Citations for Mouse/Rat GABA-A R beta 3 C-Terminus Antibody

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