GABA-A R beta 3 Antibody - Azide Free

Novus Biologicals | Catalog # NB300-199

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all GABA-A R beta 3 Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the cytoplasmic loop of the beta 3 subunit. Accession # P63079

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 21901840).

Specificity

Specific for the ~53 kDa beta 3-subunit of the GABAA receptor in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GABA-A R beta 3 Antibody - Azide Free

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199] - Immunostaining of mouse retina showing specific labeling of the GABAA beta3 subunit in green, calbindin in red and DNA in blue.
Western Blot: GABA-A R beta 3 Antibody [NB300-199]

Western Blot: GABA-A R beta 3 Antibody [NB300-199]

GABA-A-R-beta-3-Antibody-Western-Blot-NB300-199-img0010.jpg
Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry: GABA-A R beta 3 Antibody [NB300-199]

GABA-A-R-beta-3-Antibody-Immunohistochemistry-NB300-199-img0011.jpg
Western Blot: GABA-A R beta 3 Antibody [NB300-199]

Western Blot: GABA-A R beta 3 Antibody [NB300-199]

Western Blot: GABA-A R beta 3 Antibody [NB300-199] - Rat brain lysate showing specific immunolabeling of the 53 kDa Beta(3)-subunit of the GABA(A)-R
Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry-Paraffin: GABA-A R beta 3 Antibody [NB300-199] - Paraffin-embedded, formalin-fixed mouse head section. WB image submitted by a verified customer review.
Immunohistochemistry-Frozen: GABA-A R beta 3 Antibody [NB300-199]

Immunohistochemistry-Frozen: GABA-A R beta 3 Antibody [NB300-199]

GABA-A-R-beta-3-Antibody-Immunohistochemistry-Frozen-NB300-199-img0009.jpg

Applications for GABA-A R beta 3 Antibody - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:300

Simple Western

1:100

Western Blot

1:1000
Application Notes
Use in Immunohistochemistry free-floating reported in scientific literature (PMID: 26592770). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID: 17021187). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 23430456). See Simple Western Antibody Database for Simple Western validation: Human Brain lysate at 0.5 mg/ml as sample; separated by size; antibody dilution of 1:100; observed molecular weight was 48 kDa;matrix was 12-230 kDa; detected by Chemiluminescence.

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-199 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/ml BSA, 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GABA-A R beta 3

GABA (A) receptors are ligand-gated chloride channels that play a role as inhibitory neurotransmitters. They are known targets for certain classes of environmental and pharmaceutical compounds because a number of drugs interact with binding sites on GABA (A). Some of these drugs include benzodiazepines, anticonvulsants, and anesthetics. GABA (A) Receptors are comprised of subunits and these are further classified into 3 major groups (alpha, beta, and gamma). The subunits determine the pharmacological characteristics.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-3 Polypeptide

Alternate Names

GABAAR beta 3, GABAARb3, GABRB3

Entrez Gene IDs

2562 (Human); 24922 (Rat)

Gene Symbol

GABRB3

UniProt

P63079 (Rat)

Additional GABA-A R beta 3 Products

Product Documents for GABA-A R beta 3 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GABA-A R beta 3 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

GABA Receptors

Citations for GABA-A R beta 3 Antibody - Azide Free

Customer Reviews for GABA-A R beta 3 Antibody - Azide Free (2)

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Customer Images

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  • GABA-A R beta 3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Mouse brain
    Species: Mouse
    Verified Customer | Posted 08/22/2018
    IHC was performed on paraffin-embedded, formalin-fixed mouse head sections.
    GABA-A R beta 3 Antibody - Azide Free NB300-199
  • Name: Corey.
    Application: Western Blot
    Sample Tested: Mouse forebrain lysate
    Species: Mouse
    Verified Customer | Posted 04/04/2013
    GABA-A R beta 3 Antibody - Azide Free NB300-199

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Protocols

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FAQs for GABA-A R beta 3 Antibody - Azide Free

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  • Q: Could you please confirm the concentration for cat# NB300-199? The lot is WS712G.

    A: The concentration of that lot # is 0.15 mg/mL.

  • Q: Could you please provide the immunogen sequence for Anti-GABA A Receptor beta 3 antibody and Anti-GABA A Receptor beta 2 antibody, catalog number NB300-199 and NB300-198 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived? Also, please let me know if this information can be made public. Thank you for your help in this matter.

    A: The immunogen for NB300-199 falls within 340-420 amino acids, while the immunogen for NB300-198 falls within the range of 327-451.

  • Q: Which species is the sequence from?

    A: This sequence came from rat.

  • Q: Could you please confirm the concentration for cat# NB300-199? The lot is WS712G.

    A: The concentration of that lot # is 0.15 mg/mL.

  • Q: Could you please provide the immunogen sequence for Anti-GABA A Receptor beta 3 antibody and Anti-GABA A Receptor beta 2 antibody, catalog number NB300-199 and NB300-198 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived? Also, please let me know if this information can be made public. Thank you for your help in this matter.

    A: The immunogen for NB300-199 falls within 340-420 amino acids, while the immunogen for NB300-198 falls within the range of 327-451.

  • Q: Which species is the sequence from?

    A: This sequence came from rat.

  • Q: Could you please confirm the concentration for cat# NB300-199? The lot is WS712G.

    A: The concentration of that lot # is 0.15 mg/mL.

  • Q: Could you please provide the immunogen sequence for Anti-GABA A Receptor beta 3 antibody and Anti-GABA A Receptor beta 2 antibody, catalog number NB300-199 and NB300-198 at your earliest convenience? If the exact sequence is not available, could you please tell me a broader range of amino acids or the region of the peptide from which it is derived? Also, please let me know if this information can be made public. Thank you for your help in this matter.

    A: The immunogen for NB300-199 falls within 340-420 amino acids, while the immunogen for NB300-198 falls within the range of 327-451.

  • Q: Which species is the sequence from?

    A: This sequence came from rat.

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